Abstract:

Objective To compare the effects of two ELISA kits on IgG antibody detection of human Echinococcus granulo? sus. Methods A Total of 134 sera of patients with echinococcosis，paragonimiasis westermani，clonorchiasis sinensis，schistoso? miasis japonica，and cysticercosis cellulosae，and normal persons were detected by two IgG ELISA kits produced by different com? panies. Furthermore，the specificity，sensitivity and cross reactivity were counted and analyzed statistically. Results The sensi? tivity and specificity were extremely high of the two kits as 100.00%. The cross?reactivity rates were 25.00%（paragonimiasis wes? termani），26.09%（clonorchiasis sinensis），10.00%（schistosomiasis japonica），and 87.5%（cysticercosis），respectively，by us? ing the kit produced by the Combined Company in Shenzhen；the cross?reactivity rates were 5.00%（paragonimiasis westermani）， 13.04%（clonorchiasis sinensis），20.00%（schistosomiasis japonica），and 93.75%（cysticercosis）respectively，by using the kit produced by Haitai Company in Zhuhai. In addition，there was a significant difference of Paragonimus westermani detection（P < 0.05），but the rests had no statistically significant differences（all P > 0.05）between the two kits. Conclusion Both ELISA kits on IgG antibody detection of human Echinococcus granulosus have the advantages of a high sensitivity，specificity，convenience and high?speed. However，it is also in urgent need to further solve the cross?reactivity of Echinococcus granulosus with other para? sites，in order to improve the accuracy of early diagnosis.

Key words: Echinococcus granulosus； IgG antibody； Enzyme?linked immunosorbent assay（ELISA）； Kit；Cross?reaction

摘要： 目的 观察不同厂家生产的2种棘球绦虫IgG抗体检测试剂盒的检测效果。 方法 采用2种不同厂家生产的棘 球绦虫IgG抗体检测试剂盒检测相同数量的棘球蚴病人、 正常人血清， 以及卫氏并殖吸虫、 华支睾吸虫、 日本血吸虫和猪囊 尾蚴病人血清， 统计2种方法的特异性、 敏感性和交叉反应性。 结果 2个厂家生产的棘球绦虫IgG抗体检测试剂盒的特 异性和敏感性均为100％。深圳康百得公司生产的试剂盒与卫氏并殖吸虫病、 华支睾吸虫病、 日本血吸虫病和猪囊尾蚴病 的交叉反应率分别为25.00％、 26.09％、 10.00％和87.50％； 珠海海泰公司生产的试剂盒的交叉反应率分别为5.00％、 13.04％、 20.00％和93.75％。2种试剂盒间， 卫氏并殖吸虫病检出率差异有统计学意义 （P ＜ 0.05）， 其他检出率差异均无统 计学意义 （P均 ＞ 0.05）。结论 2个厂家生产的棘球绦虫IgG抗体检测试剂盒均具有敏感性高、 特异性强、 简便快速等优 点； 但需进一步解决与其他寄生虫病的交叉反应问题， 以提高早期诊断的准确性。

关键词: 棘球绦虫； IgG抗体； 酶联免疫吸附试验； 试剂盒； 交叉反应