Abstract:

of Schistosoma japonicum in vitro and compare its efficacy with praziquantel. Methods ICR mice were infected
with the cercariae of S. japonicum by the patching abdominal method. The livers of the mice were grinded，screened，and then the
eggs of S. japonicum were obtained 42 days post?infection. The miracidia were hatched by using the eggs，and the cercariae were
obtained by using the infected Oncomelania snails on the light. The eggs，miracidia and cercariae of S. japonicum were incubated
with 0，1，2，3，4，5，6，7，8 μg/ml PRS for different time，and praziquantel（PZQ）was used as the control. Results PRS sup?
pressed the hatching rates of eggs for 24 h slightly superior to that of the control drug PZQ at different concentrations，especially in
the 4 μg/ml concentration. After the miracidia were incubated with 1，2，3，4，5，6，7，8 μg/ml PRS for 30 min，the dead rates
of miracidia were 13.47，26.05，60.99，90.84，100，100，100，100%，respectively. After the cercariae were incubated with 1，
2，3，4，5，6，7，8 μg/ml PRS for 30 min，the dead rates of cercariae were 5.32，18.81，44.7，76.87，98.28，100，100，100%，
respectively. PRS showed time? and dose?dependent mortality effects on the miracidia and cercariae of S. japonicum. Conclusion
PRS has the effects against eggs，miracidia，cercariae of S. japonicum in vitro，and it may become a new anti?schistosome agent.

Key words: Schistosoma japonicum；Pulsatilla chinensis（Bunge）Regel saponins（PRS）；Egg；Miracidium；Cercaria

摘要：

目的 观察白头翁总皂苷（Pulsatilla chinensis（Bunge）Regel saponins，PRS）对日本血吸虫虫卵、毛蚴、尾蚴的杀灭效果，为白头翁总皂苷作为抗血吸虫新药提供理论和实验依据。方法 采用尾蚴腹部贴片法感染ICR小鼠，感染后42 d的鼠肝脏经研磨、过筛获得虫卵，毛蚴由虫卵孵化，尾蚴由阳性感染钉螺光照逸出，采用0、1、2、3、4、5、6、7、8 μg/ml 的PRS药液分别于不同时间作用虫卵、毛蚴、尾蚴。结果 不同浓度PRS及对照药物吡喹酮（PZQ）对日本血吸虫虫卵作用24 h的孵化结果显示，PRS对虫卵孵化的抑制效果略优于PZQ，尤其在4 μg/ml浓度时，日本血吸虫虫卵对PRS的作用更敏感。1、2、3、4、5、6、7、8 μg/ml 的PRS药液作用于毛蚴30 min后，毛蚴死亡率分别为13.47%、26.05%、60.99%、90.84%、100%、100%、100%、100%。作用于尾蚴30 min后，尾蚴的死亡率分别为5.32%、18.81%、44.7%、76.87%、98.28%、100%、100%、100%。毛蚴、尾蚴的死亡率对PRS的作用时间和浓度有一定依赖性。 结论 体外实验显示PRS对日本血吸虫虫卵、毛蚴、尾蚴均有杀伤作用，有望成为新的抗血吸虫药物。

关键词: 日本血吸虫；白头翁皂苷；虫卵；毛蚴；尾蚴