Abstract:

Objective To study the influences of Euphorbia helioscopia L. on glycogen contents of Oncomelania hupensis so as to explore its molluscicidal mechanism. Methods The different polar factions of Euphorbia helioscopia L. were separated by us? ing the systemic solvent segregation method，and then its molluscicidal activity was detected according to the Laboratory Final Mil? luscicides Screening Method issued by WHO. The glycogen contents of soft tissues of Oncomelania hupensis snails treated by Eu? phorbia helioscopia L. ethanol extracts were determined by the anthrone method. Results After being immersed for 48 h with Eu? phorbia helioscopia L. ethanol extracts，the death rates of the snails at the concentrations of 400 mg/L and 800 mg/L were 50.43% and 77.50% respectively，while after 96 h，the death rates at the above concentrations were both 100%. The glycogen contents of the snails gradually reduced with the increase of the drug concentrations，the glycogen content decreased to the lowest while the concentration was 800 mg/L，and the glycogen content decreased by 69.49% compared with that of the control group. Conclu? sion The Euphorbia helioscopia L. ethanol extracts can significantly decrease the glycogen content of Oncomelania snails and the extracts have an adequate molluscicidal activity.

Key words: Euphorbia helioscopia L.；Oncomelania hupensis；Molluscicidal effect；Glycogen

摘要：

目的观察泽漆对钉螺软体组织糖原的影响，以探讨其灭螺机制。方法采用系统分离法分离泽漆极性部位，按照WHO推荐的“杀螺剂实验室终筛方法”中浸泡方法进行浸杀灭螺试验；蒽酮比色法测定经泽漆乙醇提取物处理后钉螺软体组织的糖原含量变化。结果钉螺经泽漆乙醇提取物400、800 mg/L作用48 h，钉螺死亡率分别为50.43%、77.50%；作用96 h，钉螺死亡率均达100%。钉螺体内糖原含量随药物浓度的增大而逐步降低，800 mg/L时糖原下降最明显，较对照组含量下降69.49%。结论泽漆乙醇提取物能够显著降低钉螺体内的糖原含量，具有较好的灭螺效果。

关键词: 泽漆；钉螺；杀螺效果；糖原