Abstract:

Objective To evaluate the effect of transdermal agent of PZQ on infection of Schistosoma japonicum in different developmental stages and the early period of re?infection. Methods All Kunming mice in the experiment groups were infected with 40 ± 2 Schistosoma japonicum cercariae. The mice which were infected once and re?infected were treated by abdominal trans? dermal agent of praziquantel. Control groups were set for all of the experiment groups. All of the mice were dissected 42 d after the infection, and the worm reduction rate, egg reduction rate and miracidium hatching reduction rate were calculated. In addition, the dynamic changes of IgG antibody in sera of the mice were detected by ELISA in different time of primary infection and re?infection. Results The worm reduction rates of 1, 7, 14, 21 and 28 d after the infection were 48.9%, 0%, 28.8%, 84.3% and 70.2%, respec? tively, and those of 1 d and 14 d after re?infection were 85.6% and 90.8%, respectively. After the primary infection, the specific IgG level gradually increased with the prolongation of time, and the ratio of P/N of specific anti?ESA of IgG was significantly raised after re?infection. Conclusion The transdermal agent of praziquantel is effective to Schistosoma japonicum at developmental stag? es, and the effect to schistosomula at early period of re?infection is more significant.

Key words: Schistosoma japonicum, Praziquantel, Transdermal treatment, Effect

摘要：

目的 观察吡喹酮透皮剂对不同发育阶段日本血吸虫感染和再感染早期的治疗效果。方法 各试验组昆明小鼠均感染日本血吸虫尾蚴40±2条， 对一次感染和再感染早期的昆明小鼠进行透皮治疗。各试验均设对照组， 感染后42 d 解剖冲虫， 计算减虫率、 减肝卵率和减毛蚴孵化率， 评价透皮剂的治疗效果。ELISA检测初次感染和再感染不同时期IgG 抗体水平的动态变化。结果 首次感染后1、 7、 14、 21、 28 d减虫率分别为48.9%、 0、 28.8%、 84.3%、 70.2%； 再感染后1 d和 14 d减虫率分别为85.6%和90.8%。首次感染后随感染时间延长， 小鼠体内特异性IgG抗体水平逐渐上升； 再感染后小鼠体内针对小鼠抗虫卵可溶性抗原特异性IgG 阳性和阴性比值明显上升。结论 吡喹酮透皮剂对不同发育阶段的日本血吸虫均有不同程度的杀灭作用， 对再感染早期童虫的减虫效果更好。

关键词: 日本血吸虫； 吡喹酮；透皮治疗； 效果