Abstract:

Objective To study the skin inflammation responses induced by heat shock protein 70 (rSj648/hsp70) from Schis? tosoma japonicum in BALB/c mice. Methods BALB/c mice were injected with 20 μg of LPS in abdominal skin or 100 μg of rSj648/hsp70，meanwhile，the PBS?treated group was set as blank. On days 1，2，4，7 after the immunization，dynamic changes of inflammation were observed by staining with HE；and the IFN?γ mRNA expression was detected by real ? time PCR. Results On day 1，the inflammation of the skins derived from mice injected with LPS was obviously，and then fell off gradually after day 1. Compared with the LPS?treated group，the inflammation responses induced by Sj648/hsp70 were longer and more intensive until day 7. The real?time PCR showed that the IFN?γ mRNA was expressed highly in both LPS?treated group and Sj648/hsp70?treated group，then it fell off in the LPS?treated group，but the IFN?γ mRNA was still expressed higher in the Sj648/hsp70?treated group until day 7. Conclusions Heat shock protein 70 (rSj648/hsp70) induces high protection against schistosome?infection contribut? ing to predominant Th1 reaction and the correlation with high expression of IFN?γ.

Key words: Schistosoma japonicum；Heat shock protein 70；Inflammation response；Skin； Immune response； Mouse

摘要：

目的 目的 观察重组日本血吸虫热休克蛋白70 （rSj648/hsp70） 诱导BALB/c小鼠皮肤炎症反应的变化情况。 方法 方法 BALB/c小鼠经腹部皮内分别免疫注射LPS （20 μg/只）、 rSj648/hsp70 （100 μg/只）， 同时设PBS处理组为空白对照。通过苏木素伊红 （HE） 染色观察免疫后第1、 2、 4、 7天小鼠腹部皮肤的炎症反应变化， 并利用Real?time PCR方法检测不同时间点各组小鼠皮肤组织IFN?γ mRNA的表达水平。 结果 结果 通过HE染色观察发现LPS组小鼠腹部皮肤组织在免疫后第1天炎症细胞浸润明显， 之后炎症反应开始逐渐减轻； 与LPS组相比较， rSj648/hsp70组小鼠腹部皮肤炎症反应强度大， 持续时间长，直至免疫后第7天炎症反应仍明显。Real?time PCR结果显示， 免疫后第1天， LPS组和rSj648/hsp70组小鼠皮肤组织的IFN? γ mRNA表达水平均升高， 之后LPS组逐渐降低， 而rSj648/hsp70组直至免疫后第7天仍持续高表达。 结论 结论 rSj648/hsp70 能使小鼠皮肤出现较强的以诱导产生IFN?γ为主的Th1优势免疫应答， 进一步提示了HSP70高表达可能与致弱尾蚴诱导宿主产生抗感染免疫力有关。

关键词: 日本血吸虫； 热休克蛋白70； 炎症反应； 皮肤； 免疫应答； 小鼠