Abstract:

Objective To observe the effects of recombinant Toxoplasma gondii P30 antigen and soluble antigen for IgM detection of toxoplasmosis. Methods Recombinant Toxoplasma gondii P30 antigen and soluble antigen were coated on two nitrocellulose membranes (NC) respectively. They were combined with the corresponding antibodies in the testing sera. The gold?labeled rabbit anti?human IgM(s) were detected for corresponding antibodies and the positive visualized. The sera of toxoplasmosis, rheumatoid arthritis, Mycoplasma pneumonia, schistosomiasis patients and healthy persons were tested. Results A total of five kinds of 166 serum samples were detected with dot immunogold filtration assay (DIGFA) of the two antigens respectively. In 38 serum samples of Toxoplasma gondii infection, the sensitivity of P30?DIGFA was 92.1% (35/38) and soluble antigen?DIGFA 81.6% (31/38). There was no significant difference (P > 0.05). Totally 63 normal serum samples showed negative, so the specificity was 100%. In 30 serum samples of rheumatoid arthritis patients, 2 cases were positive in P30?DIGFA and 3 positive in soluble antigen?DIGFA. Totally 25 serum samples of schistosomiasis patients and 10 serum samples of Mycoplasma pneumonia patients showed negative. Conclusion Recombinant Toxoplasma gondii P30 antigen may be developed into clinical diagnostic kits for detection of early or acute toxoplasmosis.

Key words: Toxoplasma gondii, Recombinant P30 antigen, Soluble antigen, Dot immunogold filtration assay (DIGFA)

摘要：

目的 观察弓形虫P30重组抗原及可溶性抗原检测弓形虫病IgM的效果。方法 方法 建立斑点免疫金渗滤法 （DIG? FA）。采用2种抗原分别包被在两条硝酸纤维素膜 （NC） 上， 与待检血清中的相应IgM抗体结合， 通过金标记兔抗人IgM直接显色， 以检测弓形虫病IgM。2种抗原的DIGFA分别检测弓形虫IgM抗体阳性、 类风湿、 支原体肺炎、 血吸虫病人和正常人血清。结果 结果 两种抗原建立的DIGFA检测试剂共检测5种血清样本166份， 其中弓形虫感染IgM抗体阳性病人血清38 份， 弓形虫P30?DIGFA敏感性为92.1% （35/38）， 可溶性抗原?DIGFA敏感性为81.6% （31/38） ,差异无统计学意义(P ＞0.05)。正常人群对照血清63份， 检测全部阴性， 特异性为100%。检测类风湿病人血清30份、 血吸虫病人血清25份及支原体肺炎病人血清10份， 仅类风湿病人血清出现阳性交叉反应， 其中弓形虫P30?DIGFA 阳性2例， 可溶性抗原?DIGFA 阳性3例。结 结论 论 弓形虫P30重组抗原检测弓形虫病人血清IgM的DIGFA试剂可用于临床早期或急性期弓形虫病的诊断。

关键词: 弓形虫, P30重组抗原, 可溶性抗原, 斑点免疫金渗滤法