Abstract:

Objective To observe the changes of alveolar macrophage (AM) phagocytosis and the levels of TNF-α and IL-6 in AM supernatants and bronchoalveolar lavage fluid （BALF） of rats with Pneumocystis pneumonia (PCP). Methods Wistar rats were injected with dexamethasone intramuscularly and continually to establish the model of PCP, the AM phagocytosis and levels of TNF-α and IL-6 in AM supernatants and BALF of rats with PCP were detected, meanwhile the normal controls were set. Results The phagocyting percentage [（20.61±2.04）%] and phagocyting index（0.25±0.21）of the PCP group were significantly lower than those [（25.45±3.1）% and （0.31±0.16）] of the control group （P<0.05）, TNF-α (16.84±0.86) pg/ml and IL-6 (1.05±0.19) pg/ml in BALF of the PCP group were significantly higher than those [(12.48±0.84) pg/ml and （0.86±0.11）pg/ml] of the control group （P<0.05）, but there were no significant differences for the levels of TNF-α and IL-6 in AM supernatants between the two groups （P＞0.05）. Conclusion AM phagocytosis reduces evidently of PCP rats, but the levels of TNF-α and IL-6 in BALF rise evidently.

Key words: Pneumocystis pneumonia (PCP), Alveolar macrophage (AM), Phagocytosis, TNF-αand IL-6, Rat

摘要：

目的 观察肺孢子菌肺炎(PCP)大鼠肺泡巨噬细胞（AM）吞噬功能及支气管肺泡灌洗液(BALF)、AM培养上清TNF-α、IL-6的变化。方法 Wistar大鼠连续肌肉注射地塞米松建立免疫抑制PCP模型，检测大鼠AM吞噬功能及BALF、AM培养上清TNF-α、IL-6水平，同时设正常对照组。结果 PCP模型组大鼠AM吞噬鸡红细胞百分率为（20.61±2.04）%，吞噬指数为0.25±0.21，均明显低于对照组的（25.45±3.1）%和0.31±0.16（P均<0.05）。?BALF中TNF-α为(16.84±0.86) pg/ml，IL-6为(1.05±0.19) pg/ml，均明显高于对照组的(12.48±0.84) pg/ml和(0.86±0.11）pg/ml（P均<0.05）；AM培养上清中TNF-α、IL-6差异均无统计学意义（P均＞0.05）。结论 使用糖皮质激素造成大鼠免疫抑制诱发PCP后，大鼠AM吞噬功能降低，BALF中TNF-α、IL-6升高。

关键词: 肺孢子菌肺炎, 肺泡巨噬细胞, 吞噬功能, TNF-α, IL-6, 大鼠