Abstract:

 Objective   To compare the growth of Plasm,odium vivax in A规opheles sinensis(An., s)
and Anopheles anthropop矗agus (An. a) during the periods of malaria clinic attack and diapause.
Methods  The blood samples of patients during the clinic attack and diapause of vivax malaria pa-
tients in the vivax epidemic area in China were collected, feeding the mosquitoes of An. s and An. a
by using the artificial membrane feeding system /咒 vivo in the lab, and the mosquitoes were dissect-
ed during the day 7-9th and 14th after the infection and the oocysts and sporozoites in the stomach
and gland of the mosquitoes were counted, respectively. Results  The oocyst positive rate in An.s
fed by Plasmodium vivar during the fever stage was lower than that in non-fever stage, the sporo-
zoite positive rate in An. a fed by Plasmodium vivax during the fever stage was lower than that in
non-fever stage. The positive mosquito rate with oocyst and sporozoite infected by Plasmodiu,m v/-
var in the fever stage to An. s and An., a were lower than those in non-fever stage.  The infective
 sporozoite intensity of An.s fed by Plasmodium vivar in the fever stage was lower than that in non-
 fever stage, but the reverse result was found to An. a. Conclusion   There is a significant difference
between the periods of malaria clinic attack and diapause of the oocyst and sporozoite infection to An. s and An,. a.

Key words:  , Artificial blood feeding, An.opheles sin.ensis, Anopheles anthropophagus, Period of clinic attack, Period of diapause

摘要：

目的  比较临床发作期与间歇期间日疟原虫在中华按蚊和嗜人按蚊体内发育的差异。方法在我国间日疟流行区分别采集临床发作期与间歇期间日疟病例的血样，采用体外人工膜饲感染系统在实验室同时体外人工感染中华按蚊和嗜人按蚊，在感染后7～9 d和14 d分别解剖蚊胃和唾液腺，并检测蚊体内的卵囊和子孢子数。结果临床发作期间日疟原虫感染中华按蚊的卵囊阳性率和阳性蚊比率、子孢子阳性蚊比率和感染度均低于间歇期间日疟原虫感染，临床发作期间日疟原虫感染嗜人按蚊的子孢子阳性率、阳性蚊比率和卵囊阳性蚊比率均低于间歇期间日疟原虫感染，而子孢子感染度则高于间歇期疟原虫感染。结论临床发作期与间歇期间日疟原虫体外人工感染中华按蚊、嗜人按蚊卵囊和子孢子有差异

关键词:  人工感染；中华按蚊；嗜人按蚊；间歇期；发作期