Chin J Schisto Control

   

Effect of ICOS signaling on CD154/CD40 expressions in mice infected with Schistosoma japonicum

WANG Yu1, CAI Ru1, XIA Chao-ming2   

  1. 1 Department of Basic Medical Sciences|Medical College|Anhui University of Science &|Technology|Huainan 232001|China;
    2 Department of Parasitology|Medical College of Soochow University|Suzhou 215123|China
  • Received:2015-01-04 Online:2015-03-31 Published:2015-03-31

ICOS信号对感染日本血吸虫小鼠CD154/CD40表达的影响

王瑜1|蔡茹1|夏超明2   

  1. 1 安徽理工大学医学院基础医学系(淮南232001);2 苏州大学医学部病原生物学系
  • 通讯作者: 夏超明
  • 作者简介:王瑜|男|博士|副教授。研究方向:病原感染免疫学
  • 基金资助:

    国家自然科学基金(81171603);安徽理工大学博士科研基金(11046)

Abstract:

Objective  To explore the effect of ICOS signaling on the CD154/CD40 expressions and immunopathology in mice infected with Schistosoma japonicum. Methods  ICOS transgenic(ICOS?Tg)mice and wildtype FVB/NJ mice were used as experimental schistosomiasis models. The expressions of CD154 and CD40 on splenocytes and on inflammatory cells around granulomatous infiltration of the liver in the mice infected with S. japonicum were detected by flow cytometry and immunohistochemical staining on the day before infection(0 week)and in 4,7,12,16 and 20 weeks post?infection,respectively. Results    Compared with the wildtype FVB/NJ mice,the expressions of CD154 on CD4+ T splenocytes and of CD40 on CD19+ B splenocytes in the ICOS?Tg mice significantly increased in 12 and 16 weeks post?infection(P < 0.05). Moreover,the expressions of CD40 and CD154 on inflammatory cells around granulomatous infiltration in the liver of the ICOS?Tg mice were significantly higher than those of the wildtype FVB/NJ mice in 7,12,16 and 20 weeks post?infection(P < 0.05). The volumes of liver egg granulomas of the ICOS?Tg mice were significantly bigger than those of the wildtype mice(P < 0.05 or P < 0.01). Conclusions  In ICOS?Tg mice infected with S. japonicum,the ICOS signaling has a regulatory effect on CD154/CD40 expressions,and may play an important role in the hepatic egg granuloma formation of schistosomiasis.

Key words: Schistosoma japonicum, ICOS signaling, CD154/CD40, Egg granuloma, Transgenic mice

摘要:

目的  探讨可诱导共刺激分子(Inducible costimulator,ICOS)信号对感染日本血吸虫小鼠的CD154/CD40表达及免疫病理的影响。方法  建立ICOS转基因(ICOS transgenic,ICOS?Tg)小鼠及野生型FVB/NJ小鼠日本血吸虫病模型,应用流式细胞术、免疫组化技术分别检测感染前后的小鼠脾淋巴细胞与肝脏虫卵肉芽肿周围炎性浸润细胞CD154、CD40表达水平。应用HE染色法观察小鼠肝脏虫卵肉芽肿病变动态变化。结果  与野生型FVB/NJ小鼠相比,ICOS?Tg小鼠脾淋巴细胞CD154、CD40表达水平升高,在感染后12、16周差异有统计学意义(P 均< 0.05);ICOS?Tg小鼠肝脏虫卵肉芽肿
炎性浸润细胞CD154、CD40表达亦显著升高,在感染后7、12、16、20周差异有统计学意义(P 均< 0.05)。且ICOS?Tg小鼠肝脏虫卵肉芽肿体积显著大于野生型小鼠(P < 0.01或0.05)。结论  在日本血吸虫感染中,ICOS信号对CD154/CD40表达具有一定的调控作用,在免疫病理中起重要调控作用。

关键词: 日本血吸虫, ICOS信号, CD154/CD40, 虫卵肉芽肿, 转基因小鼠

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