Abstract:

Objective To evaluate the potency of NP30 antibody detection for the diagnosis of schistosomiasis japonica in the mountainous endemic spots in Yunnan Province. Methods A total of 506 inhabitants in the mountainous schistosomiasis endemic regions of Yunnan Province were ex- amined by using the stool hatching test for parasitological stool examination; the sera samples from 506 villagers were tested with NP30 antibody detection and SEA-EI.ISA for immune examination respectively, and the sensitivity of NP30 antibody detection was estimated . Their specificity was determined by testing 100 sera from nonepidemic scenes. Results The positive coincidence of stool hatching test with NP30 antibody detection and SEA-ELISA were 87. 80%(144/164)and 84. 76% (139/164) respectively, and their specificity as 96. 00%(96/lOO)and 94. oo% (94/100), with no significant difference between them (P>O. 05). However, in the negative sera from stool hatching test, the detection rate was 44.15% (151/342) by NP30 antibody detection, and 70.47%(241/ 342)by SEA-ELISA, the difference was significant(P<0. 05).

Key words: Schistosomiasis japonica, Anti-idiotypic, Antibody detection, SEA-ELISA

摘要：

目的 评价日本血吸虫抗独特型抗体NP30抗体检测法在云南大山区血吸虫病流行现场的应用效果。方法 对云南大山区血吸虫病流行区的506位居民进行粪检，同时用NP30抗体检测法和血吸虫抗体ELISA检测试剂盒(SEA-ELISA)分别对其血清进行检测，评价NP30抗体检测法的敏感性；同时检测非流行区的100份血清确定其特异性。结果 NP30抗体检测法和SEA-ELISA 法的粪检阳性符合率分别为87. 80%(144/164)和84. 76%(139/164)；两者的特异性分别为96.00% (96/100)和94. 00%(94/100)，差异均无显著性(P均>0. 05)，但在粪孵阴性人群血清样本中，NP30 抗体检测法和SEA-ELISA法的检出率分别为44, 15%(151/342)和70. 47%(241/342)，差异有显著性(P<0. 05).

关键词: 日本血吸虫病；抗独特型抗体；抗体检测；可溶性虫卵抗原一酶联免疫吸附试验