Chin J Schisto Control ›› 2007, Vol. 19 ›› Issue (4): 285-288.

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Molluscicidal experiment of endophytes from Pseudolarix kaempferi Gord

Guo Shang-bin, He Jia, Cheng Xi, Zhong Nan   

  • Online:2013-01-06 Published:2013-01-14

金钱松内生真菌杀螺活性菌株筛选

郭尚彬|陈钧|何佳|承曦|仲楠   

  1. 江苏大学药学院生药学教研室(镇江212013)
  • 作者简介:郭尚彬(19 82 -)|男|硕士研究生。研究方向:药用植物内生真菌活性代谢产物筛选及新药

Abstract:

 Objective  To screen 18 endophytes from Pseudolarir kaempferi Gord for molluscici-
dal effect and identify them by morphology. Methods   Molluscicidal tests were performed according
to the immersion test suggested by WHO and the strain screened was identified by the slide culture.
Results   The mortality rates of snails immersed by JJ18 broth salified (pH = 7) were 26.7%,
76. 7% and l00. 0% for 24,48 h and 72 h, respectively, and 53. 3% and 86. 7% in 5% and 10%
concentrations of JJ18 broth, respectively. The active components were extracellular moiety of the
broth which had no acute toxicity to fish, and JJ18 strain belonged to Aspergillus. Conclusion  Ex-
tracellular moiety of endophyte JJ18 from Pseudolarir kaempferi Gord is a new resource of mollusci-
cide.

Key words:  Oncomelania snail, Endophyte, Molluscicidal effect, Pseudolarix kaempferi Gord

摘要:

目的筛选出对钉螺具有致死作用的金钱松内生真菌菌株,并进行形态学初步鉴定。方法  从药用植物金钱松的18株内生真菌中,按照WHO的杀螺剂浸泡试验法观察不同内生真菌以及不同浓度发酵液杀螺效果,并采用玻片培养法进行真菌鉴定。结果JJ18菌株发酵液的中和液(pH=7)有较高的杀螺效果,24 h钉螺死亡率达26. 7%,48 h达76. 7%,72 h达100.O%。发酵液浓度为5%时,钉螺死亡率为53.3%;浓度为10%时,钉螺死亡率为86. 7%。其杀螺活性成分主要是发酵液的细胞外产物,且对鱼无急性毒性。通过鉴定该菌属于曲霉属。结论金钱松内生真菌JJ18细胞外产物具有一定的杀螺效果,可作为筛选新型杀螺药物的资源。

关键词: 钉螺;内生真菌;杀螺作用;金钱松

CLC Number: