Chin J Schisto Control ›› 2021, Vol. 33 ›› Issue (2): 148-.

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Expression and clinicopathological significance of Bcl-2 and Bax genes in colorectal cancer patients complicated with schistosomiasis

YANG Xing-Guang, YANG Ji-Wu*, ZHAO Peng-Ju, CHENG Wei, SHI Hai-Bin, ZHANG Bin, FU Qi-Chun, LI Yi   

  1. The Second Department of General Surgery, the First Affiliated Hospital of Dali University, Dali 671000, China
  • Online:2021-04-30 Published:2021-04-30

Bcl?2Bax基因在结直肠癌合并血吸虫病患者体内表达及临床病理意义

杨兴广,杨继武*,赵鹏举,程伟,施海斌,张斌,付齐春,李逸   

  1. 大理大学第一附属医院普外二科(大理 671000)
  • 作者简介:杨兴广,男,硕士,主治医师。研究方向:普通外科疾病诊治
  • 基金资助:
    云南省教育厅科学研究基金(2019J0767)

Abstract: Objective To investigate the expression and clinicopathological significance of Bcl?2 and Bax genes in colorectal cancer (CRC) patients complicated with schistosomiasis. Methods The CRC patients receiving surgical treatment in the First Affiliated Hospital of Dali University from June 2016 to June 2020 were recruited as the study subjects, and 30 subjects were randomly sampled from the CRC patients complicated with schistosomiasis (CRC?S group) and 30 subjects were randomly sampled from the CRC patients without schistosomiasis (CRC group) using a random number table method. The cancer specimens were sampled from subjects in the CRC?S and CRC groups, and the peri?cancer specimens were sampled from subjects in the CRC group. The Bcl?2 and Bax expression was quantified in cancer and peri?cancer specimens using a real?time fluorescent quantitative PCR (qPCR) assay and immunohistochemistry at transcriptional and translational levels, and the cell apoptosis was detected in cancer specimens using HE staining. Results A total of 60 subjects were enrolled, including 30 cases in the CRC group and 30 cases in the CRC?S group. There were no significant differences between the two groups in terms of gender distribution ([χ2] = 0.271, P > 0.05), mean age (t = -0.596, P > 0.05), tumor growth pattern ([χ2] = 0.275, P > 0.05), tumor location ([χ2] = 4.008, P > 0.05), tumor invasion depth ([χ2] = 0.608, P > 0.05), degree of tumor differentiation ([χ2] = 0.364, P > 0.05), or presence of vascular metastasis ([χ2] = 1.111, P > 0.05), while significant differences were seen between the two groups in terms of histological type, presence of lymph node metastasis and TMN staging ([χ2] = 5.963, 8.297 and 5.711, all P values < 0.05). qPCR assay and immunohistochemistry quantified significantly higher Bcl?2 and Bax expression in cancer specimens from the CRC and CRC?S groups than in the peri?cancer specimens from the CRC group at both translational and transcriptional levels (all P values < 0.05), and higher Bcl?2 and lower Bax expression were seen in the cancer specimens from the CSC?S group than that from the CRC group (all P values < 0.05). In addition, the cell apoptotic rate was significantly greater in the cancer specimens in the CRC group than in the CRC?S group (42.00% vs. 23.35%; [χ2] = 41.500, P = 0.000). Conclusion Schistosomiasis may be involved in the development and progression of CRC through affecting Bcl?2 and Bax gene expression in the apoptosis signaling pathway.

Key words: Schistosomiasis, Colorectal cancer, Cell apoptosis, Bcl?2 gene, Bax gene

摘要: 目的 探讨Bcl?2和Bax基因在结直肠癌(CRC)合并血吸虫病患者体内的表达水平及其临床病理意义。方法 以2016年6月—2020年6月在大理大学第一附属医院接受手术治疗的CRC患者作为研究对象,采用随机数字表法从单纯CRC患者和CRC合并血吸虫病患者中各随机抽取30例作为CRC组和CRC?S组。收集两组患者癌组织和CRC组患者癌旁组织,采用实时荧光定量PCR法和免疫组织化学法检测样本中Bcl?2和Bax mRNA和蛋白表达水平,应用HE染色法观察并比较两组患者癌组织细胞凋亡率。结果 共纳入研究对象60例,其中CRC组和CRC?S组均30例,两组患者性别构成([χ2] = 0.271,P>0.05)、平均年龄(t = -0.596,P>0.05)、结直肠肿瘤生长方式([χ2] = 0.275,P>0.05)、肿瘤部位([χ2] = 4.008,P>0.05)、肿瘤浸润深度([χ2] = 0.608,P>0.05)、肿瘤分化程度([χ2] = 0.364,P>0.05)及肿瘤是否脉管转移([χ2] = 1.111,P>0.05)差异均无统计学意义,但肿瘤组织学类型、肿瘤是否淋巴结转移和TMN分期差异均有统计学意义([χ2] = 5.963、8.297、5.711,P均<0.05)。与CRC组患者癌旁组织相比,CRC组和CRC?S组患者癌组织中Bcl?2、Bax mRNA和蛋白表达水平均升高(P均<0.05);与CRC组相比,CRC?S组患者癌组织中Bcl?2 mRNA和蛋白表达水平均升高(P均<0.05),Bax mRNA和蛋白水平均降低(P均<0.05)。CRC组和CRC?S组患者肿瘤组织细胞凋亡率分别为42.00%和25.35%,差异有统计学意义([χ2] = 41.500,P = 0.000)。结论 血吸虫病可能通过影响细胞凋亡信号通路中Bcl?2和Bax基因表达而参与CRC发生与进展。

关键词: 血吸虫病, 结直肠癌, 细胞凋亡, Bcl?2基因, Bax基因

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