Chin J Schisto Control ›› 2020, Vol. 32 ›› Issue (3): 290-.

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Effects of copper and cadmium ions on protective enzyme activity in Oncomelania hupensis

GAO Meng-Yang1, 2, ZHANG Qin1, TANG Xiao-Niu1, ZHOU Shu-Lin1*   

  1. 1 Pathogenic Biology, Anhui College of Traditional Chinese Medicine, Wuhu 241002, China; 2 Department of Medical Parasitology, Wannan Medical College, China
  • Online:2020-05-16 Published:2020-05-16

铜和镉离子对湖北钉螺体内保护酶活力的影响

高梦阳1, 2,章琴1,唐小牛1,周书林1*   

  1. 1 安徽中医药高等专科学校(芜湖241002); 2 皖南医学院医学寄生虫学教研室
  • 作者简介:高梦阳,女,硕士研究生。研究方向:血吸虫病控制
  • 基金资助:
    安徽省自然科学基金(11040606M214)

Abstract: Objective To evaluate the effects of Cu2+ and Cd2+ at different concentrations on superoxide dismutase (SOD), catalase (CAT) and peroxidase (POD) activity in Oncomelania hupensis. Methods Cu2+? and Cd2+?containing solutions were prepared at 7 concentrations, and O. hupensis snails were exposed to the solutions for 24 h, of 15 snails in each concentration. Then, the snail body was collected following removal of the snail shell and homogenated, and the SOD, CAT and POS activities were detected in the supernatants. Results With the increase of the Cu2+ concentration, the SOD activity appeared a rise followed by a reduction in O. hupensis snails, and the CTA activity appeared a decline?rise?decline tendency, while the POD activity showed a tendency towards rise followed by decline. With the increase of the Cd2+ concentration, the SOD activity appeared a rise followed by a reduction in O. hupensis snails, and the CTA activity appeared a decline?rise?decline tendency, while the POD activity showed a decline?rise?decline tendency. Conclusion Exposure to Cu2+ and Cd2+ at high concentrations results in a decline in the activity of SOD, CAT and POD in O. hupensis at the same time.

Key words: Oncomelania hupensis, Cu2+, Cd2+, Enzyme activity

摘要: 目的 探索不同浓度Cu2+和Cd2+对湖北钉螺体内超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和过氧化物酶(POD)活力的影响。方法 按照Cu2+、Cd2+质量浓度分别设置7个浓度梯度组,每组放入15只钉螺。24 h后解剖去壳,匀浆,取上清液,分别测定各组钉螺体内SOD、CAT和POD活力。结果 随着Cu2+浓度逐渐升高,钉螺体内SOD活力呈先上升后下降的趋势,CAT活力呈下降?上升?下降趋势,POD活力呈先上升后下降的趋势。随Cd2+浓度逐渐升高,钉螺体内SOD活力呈先上升再下降的趋势,CAT活力呈下降?上升?下降的趋势,POD活力呈下降?上升?下降趋势。结论 在相同作用时间下,高浓度Cu2+和Cd2+均可使钉螺体内SOD、CAT、POD活力下降。

关键词: 湖北钉螺, 铜离子, 铬离子, 酶活力

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