Chin J Schisto Control ›› 2015, Vol. 27 ›› Issue (1): 64-.

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Experimental study on determination of viability of Schistosoma japonicum cercariae by staining

BAO Qin-lu1 |HUANG Chun-lan2Δ | CHEN Dan-dan3| 4 |WANG Xue-long3 | WANG Shao-sheng1 |TANG Xiao-niu1 |ZHOU Shu-lin1*   

  1. 1 Department of Parasitology|School of Basic Medicine|Wannan Medical College|Wuhu 241002|China;2 Clinical Laboratory of People’ s Hospital of Liuzhou|Guangxi Zhuang Autonomous Region|China;3 Department of Parasitology|Anhui Medical University|China; 4 Clinical Laboratory of Wuhu Hospital of Traditional Chinese Medicine|Anhui Province| China
  • Online:2015-03-05 Published:2015-03-06
  • Contact: ZHOU Shu?lin

染色法判别日本血吸虫尾蚴生命状态的实验研究

鲍沁露1|黄春兰2Δ|陈丹丹3|4|汪学龙3|王少圣1|唐小牛1|周书林1*   

  1. 1 皖南医学院医学寄生虫学教研室 (芜湖241002); 2 广西壮族自治区柳州市人民医院检验科; 3 安徽医科大学医学寄生虫学教研 室; 4 安徽省芜湖市中医院检验科
  • 通讯作者: 周书林
  • 作者简介:鲍沁露| 女| 硕士研究生| 研究方向: 寄生虫媒介生物学
  • 基金资助:
    安徽省自然科学基金 (11040606M214)

Abstract: Objective Objective To determine the viability of Schistosoma japonicum cercariae by staining. Methods Methods Schistosoma japonicum cercariae were stained by 0.4% trypan blue,0.5% methylene blue?eosin?borax(M.E.B),0.5% eosin,0.5% methy? lene blue and 0.05% neutral red,respectively,for 5 min,then they were observed under a stereoscopic microscope. Results Results The dead cercariae were stained in the trypan blue,M.E.B,eosin and neutral red,but unstained in the methylene blue. The vi? tal cercariae were unstained in all the five kinds of dyes. Conclusion Conclusion The staining methods by using 0.4% trypan blue, 0.5%M.E.B, 0.5% eosin and 0.05% neutral red can be used to determine the viability of S. japonicum cercariae.

Key words: Schistosoma japonicum; Cercariae; Staining method; Viability; Determination

摘要: 目的 目的 采用染色法判别日本血吸虫尾蚴的生命状态。方法 方法 以0.4%台盼蓝、 0.5%M.E.B、 0.5%伊红、 0.5%甲基 蓝、 0.05%中性红等5种染色液分别对死活日本血吸虫尾蚴染色5 min, 显微镜下观察着色情况。结果 结果 台盼蓝、 M.E.B、 伊红、 中性红4种染色液可使死尾蚴着色, 而甲基蓝染液对其不着色; 5种染液均不能使活尾蚴着色。结论 结论0.4%台盼蓝、 0.5%M.E.B、 0.5%伊红、 0.05%中性红染液染色可判别日本血吸虫尾蚴的生命状态。

关键词: 日本血吸虫; 尾蚴; 染色法; 生命状态; 判别

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