Chinese Journal of Schistosomiasis Control ›› 2022, Vol. 34 ›› Issue (1): 36-.

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Evaluation of efficiency of different anti⁃cysticercus antibody test kits for serodiagnosis of cysticercosis

ZHANG Ya⁃lan1,2, JIANG Tian⁃tian1,2, JI Peng⁃hui1,2, HE Zhi⁃quan1,2, CHEN Xi3, HONG Yang3, ZHAO Dong⁃yang1,2, DENG Yan1,2*, CHEN Wei⁃qi1,2, ZHANG Hong⁃wei1,2   

  1. 1 Henan Center for Disease Control and Prevention, Zhengzhou, Henan 450016, China; 2 Henan Medical Key Laboratory for Pathogen and Vector of Parasites, Zhengzhou, Henan 450016, China; 3 Fangcheng Center for Disease Control and Prevention, Henan Province, China
  • Online:2022-02-28 Published:2022-03-01

不同抗囊尾蚴抗体检测试剂盒用于囊尾蚴病血清学诊断效果评价

不同抗囊尾蚴抗体检测试剂盒用于 囊尾蚴病血清学诊断效果评价 张雅兰1, 2,蒋甜甜1, 2,纪鹏慧1, 2,贺志权1, 2,陈曦3,洪洋3,赵东阳1, 2,邓艳1, 2*,陈伟奇1, 2,张红卫1, 2   

  1. 1 河南省疾病预防控制中心(河南 郑州450016); 2 河南省寄生虫病原与媒介医学重点实验室(河南 郑州450016);3 河南省方城县疾病预防控制中心
  • 作者简介:张雅兰,女,硕士研究生。研究方向:寄生虫病防治
  • 基金资助:
    河南省科技计划项目(202102310453);河南省医学科技攻关计划项目(LHGJ20190683);瑞银集团慈善基金会项目(9051)

Abstract: Objective To evaluate the diagnostic efficiency of four anti⁃cysticercus IgG, IgG4 or IgM antibody test kits (enzyme⁃linked immunosorbent assay, ELISA) by different manufacturers, so as to provide insights into the epidemiological investigation and clinical detection of cysticercosis. Methods Forty serum samples from cerebral cysticercosis patients, 100 serum samples from healthy volunteers, 30 serum samples from paragonimiasis skrjabini patients, 17 serum samples from cystic echinococcosis and 19 serum samples from subcutaneous or cerebral sparganosis patients were collected and detected using anti⁃cysticercus IgG, IgG4 or IgM antibody test kits (brand A) and the anti⁃cysticercus IgG antibody test kit (brand B). The sensitivity, specificity and false negative rate of the four kits for detection of cysticercosis were estimated. Results The anti⁃cysticercus IgG, IgG4 or IgM antibody test kits (brand A) showed 95.00% (38/40), 87.50% (35/40), 7.50% (3/40) sensitivities and 98.00% (98/100), 100.00% (100/100) and 100.00% (100/100) for detection of cysticercosis, while the anti⁃cysticercus IgG antibody test kit (brand B) presented a 75.00% (30/40) sensitivity and 100.00% (100/100) specificity for detection of cysticercosis. The sensitivity for detection of cysticercosis was significantly higher by the anti⁃cysticercus IgG antibody test kit (brand A) than by the anti⁃cysticercus IgG antibody test kit (brand B) ([χ2] = 6.28, P < 0.05); however, no significant difference was seen in the specificity by two kits ([χ2] = 2.01, P > 0.05). The four ELISA kits showed overall false positive rates of 37.88% (25/66), 22.73% (15/66), 62.12% (41/66) and 15.15% (10/66) for detection of paragonimiasis, echinococcosis and sparganosis ([χ2] = 37.61, P < 0.05), and the anti⁃cysticercus IgG antibody test kit (brand A) presented the highest overall false positive rate for detection of paragonimiasis, echinococcosis and sparganosis ([χ2] = 7.56, P' < 0.008), while a higher overall false positive rate was seen for detection of paragonimiasis, echinococcosis and sparganosis by the anti⁃cysticercus IgG antibody test kit (brand A) than by the anti⁃cysticercus IgG antibody test kit (brand B) ([χ2] = 8.75, P' < 0.008). The four ELISA kits showed false positive rates of 40.00% (12/30), 16.67% (5/30), 76.67% (23/30) and 13.33% (4/30) for detection of paragonimiasis ([χ2] = 32.88, P < 0.05) and 21.05% (4/19), 26.32% (5/19), 73.68% (14/19) and 15.79% (3/19) for detection of sparganosis ([χ2] = 19.97, P < 0.05), and the highest false positive rates were found by the anti⁃cysticercus IgM antibody test kit (brand A) for detection of paragonimiasis and sparganosis (all P' < 0.008). However, the four ELISA kits showed comparable false positive rates of 52.94% (9/17), 29.41% (5/17), 23.53% (4/17) and 17.65% (3/17) for detection of echinococcosis ([χ2] = 8.24, P > 0.05). In addition, the anti⁃cysticercus IgM antibody test kit (brand A) showed false positive rates of 76.67% (23/30), 23.53% (4/17) and 73.68% (14/19) for detection of paragonimiasis, echinococcosis and sparganosis ([χ2] = 14.537, P < 0.05), with the lowest false positive rate seen for detection of echinococcosis ([χ2] = 14.537, P' < 0.014), while no significant differences were seen in the false positive rate for detection of paragonimiasis, echinococcosis and sparganosis by other three ELISA kits (all P > 0.05). Conclusions The four anti⁃cysticercus IgG, IgG4 or IgM antibody test kits exhibit various efficiencies for serodiagnosis of cysticercosis. The anti⁃cysticercus IgG antibody test kit (brand A) has a high sensitivity for serodiagnosis of cysticercosis; however, it still needs to solve the problems of cross⁃reaction with other parasitic diseases and stability.

Key words: Cysticercosis, Enzyme?linked immunosorbent assay, IgG antibody, IgG4 antibody, IgM antibody, Diagnostic efficiency

摘要: 目的 评价不同厂家生产的4种抗囊尾蚴IgG、IgG4和IgM抗体酶联免疫吸附试验(ELISA)检测试剂盒的诊断效果,为囊尾蚴病流行病学调查和临床检测提供参考。方法 采用A品牌3种抗囊尾蚴抗体(IgG、IgG4和IgM抗体)ELISA检测试剂盒以及B品牌抗囊尾蚴IgG抗体ELISA检测试剂盒,同时检测40份脑囊尾蚴病患者、100份健康人、30份斯氏并殖吸虫病患者、17份细粒棘球蚴病患者和19份皮下或脑裂头蚴病患者血清,比较不同试剂盒检测囊尾蚴病的敏感度、特异度和假阳性率。结果 A品牌抗囊尾蚴IgG、IgG4和IgM抗体ELISA试剂盒以及B品牌抗囊尾蚴IgG抗体ELISA试剂盒检测囊尾蚴病的敏感度分别为95.00%(38/40)、87.50%(35/40)、7.50%(3/40)和75.00%(30/40),特异度分别为98.00%(98/100)、100.00%(100/100)、100.00%(100/100)和100.00%(100/100);A品牌抗囊尾蚴IgG抗体ELISA试剂盒检测囊尾蚴病的敏感度高于B品牌([χ2] = 6.28,P < 0.05),两者特异度差异无统计学意义([χ2] = 2.01,P > 0.05)。4种试剂盒检测并殖吸虫病、细粒棘球蚴病、裂头蚴病患者血清总假阳性率分别为37.88%(25/66)、22.73%(15/66)、62.12%(41/66)和15.15%(10/66)([χ2] = 37.61,P < 0.05);其中A品牌抗囊尾蚴IgM抗体ELISA试剂盒检测总假阳性率最高([χ2] = 7.56,P' < 0.008),A品牌抗囊尾蚴IgG抗体ELISA试剂盒检测总假阳性率高于B品牌([χ2] = 8.75,P' < 0.008)。4种试剂盒检测并殖吸虫病患者血清假阳性率分别为40.00%(12/30)、16.67%(5/30)、76.67%(23/30)和13.33%(4/30)([χ2] = 32.88,P < 0.05),检测裂头蚴病患者血清假阳性率分别为21.05%(4/19)、26.32%(5/19)、73.68%(14/19)和15.79%(3/19)([χ2] = 19.97,P < 0.05),均以A品牌抗囊尾蚴IgM抗体ELISA试剂盒检测假阳性率最高(P' 均 < 0.008)。4种试剂盒检测细粒棘球蚴病患者血清假阳性率分别为52.94% (9/17)、29.41%(5/17)、23.53%(4/17)和17.65%(3/17),差异无统计学意义([χ2] = 8.24,P > 0.05)。A品牌抗囊尾蚴IgM抗体ELISA试剂盒检测并殖吸虫病、棘球蚴病和裂头蚴病患者血清假阳性率分别为76.67%(23/30)、23.53%(4/17)和73.68%(14/19)([χ2] = 14.537,P < 0.05),其中检测棘球蚴病患者血清假阳性率最低([χ2] = 14.537,P' < 0.014);其他3种试剂盒检测并殖吸虫病、棘球蚴病和裂头蚴病患者血清假阳性率差异均无统计学意义(P 均> 0.05)。 结论 不同囊尾蚴病免疫学诊断试剂各有优劣;A品牌抗囊尾蚴IgG抗体ELISA试剂盒敏感度较高,但需进一步解决与其他寄生虫病的交叉反应和稳定性问题。  

关键词: 囊尾蚴病, 酶联免疫吸附试验, IgG抗体, IgG4抗体, IgM抗体, 诊断效果

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