Abstract: Objective　To understand Leishmania infections among employees of China Petroleum First Construction Corporation returning from Uzbekistan, and take timely actions to prevent the spread of the epidemic. Methods　Questionnaire survey was conducted to collect screening subjects’ information. Palpation of the liver, spleen and superficial lymph nodes was performed by a physician, and the lesions on the frequently exposed skin were detected by a dermatologist. In addition, the liver and spleen sizes were measured using B?mode ultrasonography, and serum samples were collected to be subjected to an rK39?based rapid diagnostic test for detection of visceral leishmaniasis. Leishmania was detected using microscopy in the specimens sampled from the lesioned skin, and the parasites species was identified using molecular assays in parasitologically positive specimens. Results　Among the 181 employees screened, enlarged cervical lymph nodes were palpable in 6 subjects, and skin lesions were found in 12 cases. B?mode ultrasonography displayed hepatosplenomegaly in 5 cases, and rK39 test were positive in 3 serum samples. Two classical lesioned skin specimens were sampled, and Leishmania was detected in one specimen. The promastigote DNA was extracted and two fragments of 120 bp and 350 bp in sizes were amplified using PCR assay with K13A/K13B and L5.8S/LITSR primers specific to Leishmania. The two amplification products were 90% and 98% homologous to the corresponding sequences of L. major (GenBank accession numbers: EU370906.1 and FN677342.1). Conclusions　Six patients with cutaneous leishmaniasis were screened, including 2 uncured cases. One uncured case was diagnosed as imported cutaneous leishmaniasis caused by L. major infection.

Key words: Cutaneous leishmaniasis, Leishmania major, Imported case, Epidemiological investigation, Luoyang City

摘要： 目的　了解中国石油天然气第一建设公司乌兹别克斯坦务工归国员工中利什曼原虫感染情况，并及时采取相关防控措施，防止疫情扩大。方法　设计调查问卷采集筛查对象相关信息，由内科医生触诊肝、脾及浅表淋巴结，皮肤科医生检查皮肤经常暴露处皮肤损害情况，对筛查对象行B超检查肝脾大小，采集筛查对象血清，应用内脏利什曼病快速检测试纸条（rK39）检测，对于筛查中发现的有皮肤损害者，采集皮损处组织镜检利什曼原虫，病原学检测阳性组织用分子生物学方法鉴定虫种。结果　共181人接受筛查，内科检查中6人颈部可触及肿大淋巴结，皮肤科检查中12人有皮肤损害表现，B超检查提示5人肝脾肿大，rK39试纸条检测血清显示3人结果呈阳性。共采集2人份典型皮损处组织，其中1份组织病原学检测发现利什曼原虫；提取前鞭毛体DNA，用利什曼原虫属特异性引物K13A/K13B和L5.8S/LITSR分别扩增出120 bp和350 bp的片段，片段序列与硕大利什曼原虫相应序列（GenBank登录号：EU370906.1、FN677342.1）同源性分别为90%和98%。结论　筛查发现6例皮肤利什曼病患者，其中2例尚未痊愈。1例未痊愈者确诊为由硕大利什曼原虫感染引起的输入性皮肤利什曼病。

关键词: 皮肤利什曼病, 硕大利什曼原虫, 输入性病例, 流行病学调查, 洛阳市