Abstract: Objective To explore the characteristics of gene sequence of mtDNA?COⅠ of Culex pipiens pallens from different geographical regions in Shandong Province and different resistant strains from the lab and five common mosquito species， and analyze the genetic diversity of these mosquitoes. Methods Adult mosquitoes were collected from Jinan， Jining， Qingdao cities and other places in Shandong Province. The sensitive， dichlorvos?resistant， pyrethroid?resistant and propoxur?resistant strains were reared in the lab. Five species of mosquito （Cx. pipiens pallens， Cx. tritaeniorhynchus， Anopheles sinensis， Aedes albopictus， and Armigeres subalbatus） were collected from Jining City and identified in the lab. mtDNA?COⅠwas specifically amplified by PCR and sequenced. The gene sequences were compared and analyzed by the biological information systems， and the phylogenetic tree was constructed. Results The amplified mtDNA?COⅠfragments of Cx. pipiens pallens from eight different cities and four different resistant strains were 528 bp in length， with 67.4% A+T contents and two mutation sites. The nucleotide sequence homology among the different geographic strains was 99.95% and the gene sequences of the four resistant strains were the same， showing a high homogeny. The amplified mtDNA?COⅠfragments of the five species of mosquitoes were 528 bp with 408 conserved sites， 120 variable sites， 42 parsimony informative sites and 78 singleton sites. The A+T contents were between 65.7% and 68.0%. The nucleotide sequence homology among the different mosquito species was between 86.17% and 92.05%， and the molecular identification was consistent with the traditional morphological identification. The molecular phylogenetic study showed that the different species were clustered at their own branch at the species and genus levels， while genera Armigeres was distantly related to the others. Conclusion mtDNA?COⅠcould not serve as the molecular marker to analyze the population genetic variation and phylogenesis of Cx. pipiens pallens from different geographical regions and different resistant strains， but it has species and genus specificities， which could be used for the identification of the mosquito species and genus.

Key words: mtDNA?COⅠ； Mosquito； Population genetics； Shandong Province

摘要： 目的　探讨山东省不同地理株及实验室不同抗性品系淡色库蚊及5种常见蚊种的线粒体DNA细胞色素C氧化酶亚基Ⅰ（mtDNA?COⅠ）基因序列特征，分析其遗传多样性。方法　采用山东省济南、济宁、青岛等地现场采集及实验室选育的淡色库蚊成蚊，以济宁市现场采集的5种蚊（淡色库蚊、三带喙库蚊、中华按蚊、白纹伊蚊、骚扰阿蚊）作为样本，PCR扩增mtDNA?COⅠ基因并测序，分析序列特征并构建系统进化树。结果　PCR特异扩增8个不同地理株及4个实验室抗性品系的淡色库蚊mtDNA?COⅠ区域，获得长度均为528 bp的扩增片段，A+T含量为67.4%，存在两个变异位点。不同地理株淡色库蚊mtDNA?COⅠ基因同源性为99.95%，不同抗性品系淡色库蚊基因序列相同，所有蚊虫COⅠ基因具有高度保守性。5种常见蚊虫的mtDNA?COⅠ基因片段长度均为528 bp，有408个保守位点，120个变异位点，42个简约信息位点，78个单态位点，A+T含量为65.7%～68.0%，同源性分析发现蚊种间基因同源性为86.17%～92.05%，所有蚊虫的COⅠ基因分子鉴定与其形态学相吻合。系统进化关系显示，同种和同属之间呈明显的聚集，但阿蚊属成单独的分支，与其他蚊种亲缘关系较远。结论　线粒体COⅠ作为不同地理株及实验室不同品系淡色库蚊的遗传进化分子标记不够理想，但该基因具有种间和属间特异性，可用于蚊虫属和种的区分。

关键词: mtDNA?COⅠ；蚊；种群遗传；山东省