Chin J Schisto Control ›› 2017, Vol. 29 ›› Issue (3): 329-333.

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Genotype analysis of Toxoplasma gondii isolated strains from congenital teras and HIV-Toxoplasma co-infected patient in Jiangsu Province

ZHOU Yong-hua1|2* | HOU Zhao-feng2| MA Yi-wu3| WANG Xue-dong4| XU Yong-liang1| TAO Jian-ping2*   

  1. 1 Key Laboratory of National Health and Family Planning Commission on Parasitic Disease Control and Prevention| Jiangsu Provincial Key Laboratory on Parasite and Vector Control Technology| Jiangsu Institute of Parasitic Diseases| Wuxi 214064| China; 2 College of Veterinary Medicine| Yangzhou University| China; 3 First People's Hospital of Kunshan City| China; 4 Wuxi Infectious Disease Hospital| China
  • Online:2017-06-20 Published:2017-06-20
  • Contact: ZHOU Yong?hua, TAO Jian?ping

江苏省先天性畸胎弓形虫分离株和HIV-弓形虫共感染患者弓形虫基因型分析

周永华1|2*|侯照峰2|马以武3|王学东4|许永良1|陶建平2*   

  1. 1 江苏省寄生虫病防治研究所、国家卫生和计划生育委员会寄生虫病预防与控制技术重点实验室、江苏省寄生虫学分子生物学重点实验室(无锡214064);2 扬州大学兽医学院;3 江苏省昆山市第一人民医院;4 江苏省无锡市传染病医院
  • 通讯作者: 周永华,陶建平
  • 作者简介:周永华|男|博士|主任技师。研究方向:弓形虫病防治
  • 基金资助:
    “十一五”国家科技支撑计划重点项目(2010BAD04B02);教育部创新团队发展计划(IRT0978);江苏省人兽共患病学重点实验室开放课题(I1507);江苏省高校优势学科建设工程资助项目

Abstract: Objective To identify the genotype of Toxoplasma gondii isolated strains from a congenital teras (KS strain) and an HIV?Toxoplasma co?infected patient in Jiangsu Province. Methods T. gondii DNA of tachyzoites of a isolate from a congenital teras (KS strain) and blood DNA of an HIV?Toxoplasma co?infected patient in Jiangsu Province were extracted, and 11 loci were identified for the genotype by polymerase chain reaction restriction fragment length polymorphism (PCR?RFLP). Results The complete bands were obtained from the congenital teras (KS strain) and HIV?Toxoplasma co?infected patient in Jiangsu Province, and identified as T. gondii gene type I. Conclusion T. gondii gene type I may be the dominant genotype strain of T. gondii among the women who have the abnormal pregnant outcomes and HIV?Toxoplasma co?infected patients in Jiangsu Province.

Key words: Toxoplasma gondii; Genotyping; Polymerase chain reaction restriction fragment length polymorphism (PCR?RFLP); Congenital teras; HIV

摘要: 目的 鉴定分离自江苏省先天性畸胎的弓形虫虫株(KS株)和HIV?弓形虫共感染患者刚地弓形虫的基因型。方法 抽提江苏省先天性畸胎弓形虫分离株(KS株)速殖子和1例HIV?弓形虫共感染患者的全血DNA,选择11个基因位点,采用聚合酶链式反应?限制性片段长度多态性分析(PCR?RFLP)进行基因型鉴定。结果 自江苏省先天性畸胎分离的弓形虫虫株和1例HIV?弓形虫共感染患者的全血DNA样本中均获得完整分型条带,经鉴定均为弓形虫基因型Ⅰ型。结论 Ⅰ型刚地弓形虫可能是江苏地区引起妊娠异常结局妇女和HIV?弓形虫共感染患者的优势基因型虫株。

关键词: 刚地弓形虫;基因分型;聚合酶链式反应?限制性片段长度多态性分析;先天性畸胎;HIV

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