Chin J Schisto Control ›› 2017, Vol. 29 ›› Issue (2): 146-149.

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Study on cyfluthrin resistance and its mechanisms of Anopheles sinensis in Nanchang frontierport

XU Tie-long1|2| ZHANG Qiang1| LIU Lan1| ZHAO Ming-hui1| LIAO Yun1| LIAO Li-xin1| WANG Jian-jun1| ZHENG Bin2*   

  1. 1 Health?care Center| Jiangxi Entry?exit Inspection and Quarantine Bureau| Nanchang 330002| China; 2 National Institute of Parasitic Diseases| Chinese Center for Disease Control and Prevention| China
  • Online:2017-04-22 Published:2017-04-23
  • Contact: ZHENG Bin

南昌国境口岸中华按蚊氟氯氰菊酯抗药性及其机制研究

徐铁龙1|2|张强1|刘岚1|赵明惠1|廖芸1|廖立新1|王建军1|郑彬2*   

  1. 1 江西出入境检验检疫局保健中心(南昌 330002);2 中国疾病预防控制中心寄生虫病预防控制所
  • 通讯作者: 郑彬
  • 作者简介:徐铁龙|男|硕士|医师。研究方向:蚊媒抗药性及其携带病原体研究
  • 基金资助:
    质检总局科技计划应用技术研究类项目(2015IK163)

Abstract: Objective To study the cyfluthrin resistance and potential mechanisms of Anopheles sinensis in Nanchang Changbei International Airport, Nanchang City, Jiangxi Province. Methods The resistance levels of the local An. sinensis were detected by WHO drug resistance bioassay. During the bioassay, the dying mosquitos were classed as sensitive mosquitos, and the survival ones were classed as resistant mosquitos. The P450 monooxygenase activity and glutathione S?transferase activity were detected and compared between the two groups. At the same time, the death time of each sensitive mosquito was recorded, and the correlations between the death time and the P450 monooxygenase activity and glutathione S?transferase activity were analyzed, respectively. Results The bioassay mortality of the local An. sinensis was 59.5%. The differences of the P450 monooxygenase activities among the resistant mosquitos, sensitive mosquitos and laboratory sensitive mosquitos had statistical significances (F =151.89, P < 0.01), the resistant mosquitos > sensitive mosquitos > laboratory sensitive mosquitos. The differences of glutathione s?transferase activities among the three groups had no statistical significance (F = 0.72, P = 0.49). There existed positive correlation between the mosquito death time and the P450 monooxygenase activity, and the regression equation was y = 79.479 +1.512x with the correlation coefficient of 0.88, while there was no correlation between the mosquito death time and the glutathione S?transferaseactivity. Conclusion The An. sinensis in Nanchang Changbei International Airport has been resistant to cyfluthrin, and the promotion of P450 monooxygenase activity maybe one of the reasons for the resistance.

Key words: Anopheles sinensis; Cyfluthrin; Resistance; P450 monooxygenase; Glutathione S?transferase; Changbei International Airport

摘要: 目的 了解南昌昌北国际机场中华按蚊的氟氯氰菊酯抗药性,初步探索其产生机制。方法 应用WHO成蚊生测法检测当地中华按蚊抗药性水平,将生测过程中即将死亡蚊虫归为敏感蚊,存活蚊虫归为抗性蚊,检测并比较两组蚊虫P450单加氧酶、谷胱甘肽巯基转移酶(GST)活性;同时记录每只敏感蚊生测死亡时间,对生测死亡时间与P450单加氧酶、GST活性进行相关分析。结果 当地中华按蚊生测死亡率为59.5%;抗性蚊、敏感蚊、实验室敏感蚊的P450单加氧酶活性差异具有统计学意义(F = 151.89, P < 0.01),其中抗性蚊> 敏感蚊> 实验室敏感蚊;但三者GST活性差异无统计学意义(F = 0.72, P>0.05);蚊虫生测死亡时间与P450单加氧酶活性呈正相关性,相关系数为0.88,回归方程为y = 79.479 + 1.512x;生测死亡时间与GST活性不具有相关性。结论 南昌昌北国际机场中华按蚊对氟氯氰菊酯已产生抗药性,P450单加氧酶活性增强可能是其抗药性产生的机制之一。

关键词: 中华按蚊;氟氯氰菊酯;抗药性;P450单加氧酶;谷胱甘肽巯基转移酶;昌北国际机场

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