Chin J Schisto Control ›› 2016, Vol. 28 ›› Issue (5): 527-.

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Changes and significance of inflammatory cytokines in sera of mice chronically infected with larvae of Echinococcus granulosus

PAN Wei1|LI Xiang-yang1|SUN Fen-fen2|HAO Wen-ting1|QIN Su-ping1|YAN Chao1|TANG Ren-xian1|SHEN Yu-Juan3*| ZHENG Kui-yang1*   

  1. 1 Department of Pathogenic Biology and Immunology|Laboratory of Infection and Immunity|Xuzhou Medical University|Jiang? su Province|Xuzhou 221004|China;2 Experimental Teaching Center of Morphology|Xuzhou Medical University|China;3 Na? tional Institute of Parasitic Diseases|Chinese Center for Disease Control and Prevention|Key Laboratory of Parasite and Vector Bi? ology|Ministry of Health|National Center for International Research on Tropical Diseases|Ministry of Science and Technology| WHO Collaborating Centre for Tropical Diseases|China
  • Online:2016-10-25 Published:2016-10-26
  • Contact: SHEN Yu?Juan, ZHENG Kui?yang

细粒棘球蚴慢性感染阶段小鼠血清中炎症因子的变化及意义

潘伟1|李向阳1|孙芬芬2|郝文婷1|秦苏萍1|颜超1|汤仁仙1|沈玉娟3*|郑葵阳1*   

  1. 1徐州医科大学病原生物学与免疫学教研室|感染与免疫实验室(徐州221004);2徐州医科大学形态学实验教学中心;3中国疾病 预防控制中心寄生虫病预防控制所|卫生部寄生虫病原与媒介生物学重点实验室|科技部国家级热带病国际联合研究中心|世界 卫生组织热带病合作中心
  • 通讯作者: 沈玉娟,郑葵阳
  • 作者简介:潘伟|男|博士|讲师。研究方向:寄生虫分子免疫学
  • 基金资助:
    国家自然科学基金(81501762,81371842);中国博士后基金(2015M581864);江苏省教育厅高校自然科学研究基金 (15KJB310025);江苏省博士后科研资助计划(1501061A);江苏省高校优势学科建设工程资助项目(PAPD);徐州医学院优秀人才 启动基金(D2015004)

Abstract: Objective To investigate the levels of cytokines in the sera of mice chronically infected with the larvae of Echi? nococcus granulosus,and explore the mechanisms of immune regulation against parasite infection. Methods The protoscole? ces were isolated from the livers and lungs of sheep infected with E. granulosus,and then inoculated intraperitoneally to BALB/ c mice(2 000 for each mouse),to establish the mouse model of E. granulosus infection. The mice in the control group were in? jected intraperitoneally with an equal volume of PBS. The sera of both control and infected mice were collected to test the levels of multiple cytokines by using the Cytometric Bead Array(CBA)5 months post?infection. Results In contrast to the control group,the multiple cysts were found in the abdominal cavity,livers and lungs of the infected mice. Moreover,the levels of in? flammatory cytokines such as IL?17A,IL?6,IFN?γ,MCP?1,IL?12P70 and TNF?α in the sera of the infected mice were signifi? cantly higher than those in the control group(t = 2.713-9.255,all P < 0.05)while the levels of anti?inflammatory cytokine IL? 10 were elevated post?infection(t = 3.936,P < 0.001). Conclusion Higher inflammatory cytokines in the mice chronically in? fected with the larvae of E. granulosus,may benefit for the limitation of parasite growth.

Key words: Echinococcus granulosus;Protoscoleces;Inflammatory cytokine;Cytometric bead array(CBA)

摘要: 目的研究细粒棘球蚴慢性感染阶段小鼠血清中细胞因子水平的变化,探索机体对抗寄生虫感染的免疫应答 机制。方法从病羊内脏分离细粒棘球绦虫原头节,注射入BALB/c小鼠腹腔(2 000个原头节/只),建立细粒棘球蚴感染 小鼠模型,对照组小鼠腹腔注射等体积PBS。于感染5个月后,采集对照及感染组小鼠血清,采用流式液相多重蛋白定量 技术检测血清中多种细胞因子水平并分析。结果感染组小鼠腹腔、肝脏、肺脏等部位均出现多个单一性包囊;其血清 中IL?17A、IL?6、IFN?γ、MCP?1、IL?12P70、TNF?α等炎症因子水平均显著高于对照组(t = 2.713~9.255,P 均< 0.05);而抑 炎因子IL?10水平亦显著升高(t = 3.936,P < 0.001)。结论细粒棘球蚴慢性感染阶段小鼠体内炎症因子水平较高,有助 于抑制虫体生长。

关键词: 细粒棘球绦虫;原头节;炎症因子;细胞因子微球检测

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