Abstract:

Objective Objective To investigate the effect of Bacillus thuringiensis var. israelensis（Bti）on the activities of three de? toxification enzymes of Culex pipiens pallens and Aedes aegypti larvae. Methods Methods The activities of glutathione transferase，ace? tyl cholinesterase and carboxyl esterase，were detected after two kinds of mosquito larvae were treated by Bti at different time and concentrations. Results Results The activities of three detoxification enzymes of the two kinds of mosquito larvae were influenced by Bti treatment. The activity of glutathione transferase was increased after the Bti treatment，but declined significantly and in? hibited at a low level sustainably. The activity of carboxyl esterase was increased after the Bti treatment，but recovered to normal level quickly. Acetyl cholinesterase was affected slightly by the Bti treatment，shortly inhibited in the first time and then recov? ered. The active effects of the three detoxification enzymes were positively related to the concentration of Bti. Conclusion Conclusion The enzyme activities of glutathione transferase，acetyl cholinesterase and carboxyl esterase could be affected by Bti significantly.

Key words: Bacillus thuringiensis var. israelensis（Bti）； Culex pipiens pallens； Aedes aegypti； Detoxification enzymes

摘要：

目的 目的 研究苏云金杆菌以色列亚种 （Bacillus thuringrensis var. israelesis， Bti） 对蚊幼虫体内3种解毒酶活性的影响。 方法 方法 采用室内生物测定的方法， 分别测定淡色库蚊和埃及伊蚊幼虫取食Bti后体内谷胱甘肽转移酶、 乙酰胆碱酯酶和羧酸酯酶的活性。 结果 结果 Bti对蚊幼虫体内3种解毒酶活性均产生影响。蚊幼虫在Bti处理后谷胱甘肽转移酶活性显著增强又迅速下降、 后期持续抑制； 羧酸酯酶活性上升后迅速下降并恢复到正常水平； 乙酰胆碱酯酶活性受Bti影响较小， 前期受到抑制后逐步回升至正常水平。3种酶活性与Bti浓度呈正相关。 结论 结论 Bti可显著影响蚊幼体内谷胱甘肽转移酶、 乙酰胆碱酯酶和羧酸酯酶的活性。

关键词: 苏云金杆菌以色列亚种； 淡色库蚊； 埃及伊蚊； 解毒酶