Abstract: Objective To find out a quick，simple and convenient method of determining the viability of Oncomelania hupen? sis. Methods O. hupensis snails were stained for 30 minutes by 0.05% water soluble dye neutral red，0.5% methylene blue，red ink，methylene blue?eosin?borax（MEB）and 0.4% trypan blue，respectively. The soft tissue samples of the snails were observed by a stereoscopic microscope after crushing their shells. Results The vital snails were stained and the dead were unstained in the neutral red. The vital and dead snails were unstained in methylene blue. However，the vital and dead snails were stained in red ink. The partial vital and dead snails were stained in MEB. The vital snails were stained and the partial dead were stained in trypan blue. Conclusion The use of 0.05% water soluble dye neutral red is simple，rapid and accurate in determination of the viability of O. hupensis.

Key words: Oncomelania hupensis； Staining；Determination of viability

摘要： 目的 目的 寻找一种快速准确、 简便易行的鉴别湖北钉螺死活的方法。方法 方法 分别用0.05%中性红水溶性染液、 0.5% 甲基蓝染液、 红墨水、 伊红亚甲基蓝 （MEB） 染液、 0.4%台盼蓝对活、 死钉螺进行染色， 染色30 min后压碎螺壳， 体视显微镜 下观察软体组织着色情况。结果 结果 0.05%中性红可将活钉螺均染成红色， 死钉螺均未着色； 0.5%甲基蓝染色后， 活、 死钉螺 均不着色； 红墨水可将活、 死钉螺均染成红色； MEB染液可使部分活、 死钉螺着色； 0.4%台盼蓝可将活钉螺和部分死钉螺均 染成蓝色。结论 结论 使用0.05%中性红水溶性染色液对钉螺进行染色处理， 可快速、 准确、 客观地鉴别钉螺死活。

关键词: 湖北钉螺； 染色法； 死活鉴别