Chin J Schisto Control ›› 2014, Vol. 26 ›› Issue (4): 415-.

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Prediction and identification of T-cell epitopes in major group 3 allergen derived from Dermatophagoides farina

LI Na1 | LI Chao-pin1 | DIAO Ji-dong1 |ZHAO Bei-bei 1 | JIANG Yu-xin2*   

  1. 1 Department of Medical Parasitology|Wannan Medical College|Wuhu 241002|China;2 Department of Physiology|Wannan Medical College|China
  • Online:2014-08-15 Published:2014-08-12
  • Contact: JIANG Yu?xin

粉尘螨3类变应原的T细胞表位预测及鉴定

李娜1|李朝品1|刁吉东1|赵蓓蓓1|姜玉新2*   

  1. 1皖南医学院医学寄生虫学教研室 (芜湖 241002); 2皖 南医学院生理学教研室
  • 通讯作者: 姜玉新
  • 作者简介:李娜| 女| 硕士研究生。研究方向: 分子生物学与分子 免疫学
  • 基金资助:
    国家自然科学基金 (81270091、 30872367、 81172790)

Abstract: Objective To predict and identify T cell epitopes of major group 3 allergen derived from Dermatophagoides fari? na(Der f 3) . Methods The T cell epitopes of Der f 3 were analyzed through the sequence analysis by using the bioinformatics online tools. The five predicted peptides of T?cell epitopes were artificially synthesized. The spleen lymphocytes were co?cultured with the five T cell epitopes by using the modified MTT method and the levels of IL?2,IFN?γ,IL?4 and IL?5 in the supernatant of the cultures were detected by ELISA. Results Five T cell epitopes of Der f 3 were predicted and three of which could pro? mote the proliferation of the mouse spleen lymphocytes. The secretions of IL?2 and IFN?γ were significantly induced and the se? cretions of IL?4 and IL?5 were significantly decreased by three of five prediction epitopes of Der f 3:37 GDCPYQISLQSSSHFC? GG54 ,98 IYQHENYDSMTIDNDVALIKLKTPMT123 and 164 SELQRVDIDVVSREQCDQLYS184 . Conclusion Three T cell epitopes of Der f 3 have been initially identified,which lays the foundation of the diagnosis and treatment of asthma.

Key words: Dermatophagoides farina;Group 3 allergen derived from Dermatophagoides farina(Der f 3);T cell epitope; Epitope prediction; Spleen lymphocyte proliferation experiment

摘要: 目的 目的 预测并鉴定粉尘螨3类变应原 (Der f 3) 的T细胞表位。方法 方法 运用生物信息学分析软件预测Der f 3的T 细胞抗原表位, 并人工合成所预测的T细胞表位肽。采用改良MTT法, 用预测表位肽刺激致敏鼠脾淋巴细胞进行脾淋巴 细胞增殖试验; 酶联免疫吸附试验检测脾细胞培养上清液中白细胞介素?2 (IL?2)、 γ干扰素 (IFN?γ)、 IL?4及IL?5水平。结 结 果 果 成功预测了Der f 3的5个T细胞表位肽, 通过脾淋巴细胞增殖试验, 其中3个表位肽序列可促进脾淋巴细胞增殖并 刺激细胞因子IL?2和IFN?γ的分泌, 抑制细胞因子IL?4和IL?5的分泌。其序列分别为37 GDCPYQISLQSSSHFCGG54 、 98 IY? QHENYDSMTIDNDVALIKLKTPMT123 和164 SELQRVDIDVVSREQCDQLYS184 。结论 结论 初步鉴定了Der f 3变应原中3个T细 胞表位序列, 为后续过敏性哮喘的诊断和特异性免疫治疗奠定基础。

关键词: 粉尘螨; 粉尘螨3类变应原; T细胞表位; 表位预测; 脾淋巴细胞增殖试验

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