Abstract: Objective To predict and identify the linear B?cell epitopes in the major group 3 allergen derived from Derma? tophagoides farina（Der f 3） . Methods The linear B?cell epitopes of Der f 3 allergen were analyzed based on the physicochemi? cal properties of amino acids including antigenicity，surface accessibility，flexibility，hydrophilicity，beta?turn by online bioinfor? matics softwares. The eight predicted peptides of linear B?cell epitopes were artificially synthesized and incubated with three aller? gic serum pools（4 serum samples in each），which were consisted of total 12 serum samples from the allergic individuals，and the strong positive epitopes were selected. Results Eight B?cell epitopes from Der f 3 were predicted successfully. Five of eight B? cell epitopes were identified with strong IgE?binding abilities followed by specific IgE assay. The amino acid sequences of them were following：33 KAKAGDCP40 ， 86 HASGGEKIQVAEIYQHENYDSMTID110 ， 118 LKTPMTLDQTNAKPVPLPPQGSDVKVG144 ， 156QEGSYSLP163 and 199 DVANGGVDSCQGDSGGPVVD218 . Conclusions Five linear B? cell epitopes of Der f 3 allergen have been identified successfully. This result might provide a basis of the diagnosis and treatment for asthma.

Key words: Dermatophagoides farina；Major group 3 allergen derived from Dermatophagoides farina（Der f 3）；B cell epit? ope；Epitope prediction； Identify

摘要： 目的 目的 预测并鉴定粉尘螨3类变应原 （Der f 3） 中的B细胞线性表位。方法 方法 运用生物信息学技术， 从抗原性、 表 面易接近性、 柔韧性、 亲水性、 β转角等5个方面在线预测Der f 3的B细胞线性抗原表位。同时， 采用人工合成法合成相应B 细胞表位肽分别与由12份螨性过敏性哮喘患者血清组成的3个血清池共同作用， 明确其中的强阳性表位肽序列。结果 结果 成功预测了Der f 3中的8个B细胞表位肽。其中， 在过敏性患者血清与变应原特异性IgE结合试验中确认了33 KAKAG? DCP40 、 86 HASGGEKIQVAEIYQHENYDSMTID110 、 118 LKTPMTLDQTNAKPVPLPPQGSDVKVG144 、 199 DVANGGVDSCQGDSGGPV? VD218 和156 QEGSYSLP163 5个强阳性表位肽序列。结论 结论 成功确认了Der f 3变应原中5个B细胞线性表位序列， 为尘螨过敏 性哮喘的诊断和治疗奠定基础。

关键词: 粉尘螨； 粉尘螨3类变应原； B细胞表位； 表位预测； 鉴定