Abstract: Objective To evaluate the effect of Jatropha seed oils extracted by different methods on killing cercaria of Schisto? soma japonicum so as to screen the optimum process and formulations. Methods The cercaria directly contacting tests with Jatro? pha seed oils extracted by 6 different extraction processes were carried out，and the mouse immediate contacting cercaria infection trials with different formulations of Jatropha seed oil and various additives were performed. Results With 95% ethanol，the ratio of material to liquid being 1∶8，and 2 h extraction，the oil extraction rate was 30.7%. The cercaria directly contacting tests showed that 6 kinds of Jatropha seed oil killed all cercaria within 30 min. In the mouse immediate contacting cercaria infection trials，the worm declined rate of Jatropha seed oil liquid was 70.97%，and the worm declined rate of the sample added with benzyl benzoate was 58.87%，and the worm declined rate of the sample added with laurocapram was 77.42%. The worm declined rate of the sam? ples added with benzyl benzoate，dibutyl phthalate and laurocapram was 100%. Conclusion The process with 95% ethanol，the ratio of material to liquid being 1∶8，and 2 h extraction is the optimum，and the Jatropha seed oil has a good killing schistosome cercaria effect.

Key words: Schistosoma japonicum；Cercaria； Jatropha

摘要： 目的 目的 观察不同工艺提取的麻疯树籽油杀灭血吸虫尾蚴的效果， 以筛选制备麻疯树籽油的最佳工艺及配方。方 方 法 法 采用6种不同工艺提取的麻疯树籽油， 进行杀灭血吸虫尾蚴即时接触试验。采用麻疯树籽油及其与不同添加剂制成 的制剂， 进行昆明小鼠血吸虫尾蚴即时接触感染试验， 观察其抗血吸虫感染的减虫率。结果 结果 95%乙醇回流、 料液比1∶8、 2 h提取工艺出油率达30.7%。即时接触试验6种麻疯树籽油30 min内将尾蚴全部杀灭。即时接触感染试验麻风树籽油原 液减虫率达70.97%， 添加苯甲酸苄酯减虫率为58.87%， 单一添加月桂氮酮减虫率达77.42%， 添加苯甲酸苄酯、 邻苯二甲酸 二丁酯、 月桂氮酮减虫率达100%。结论 结论 95%乙醇回流、 料液比1∶8、 2 h提取工艺为麻疯树籽油最佳提取工艺。麻疯树籽 油有较好的血吸虫尾蚴杀灭作用。

关键词: 日本血吸虫； 尾蚴； 麻疯树