Chin J Schisto Control ›› 2014, Vol. 26 ›› Issue (1): 51-.

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Effect of PvMSP1 on differentiation, maturation and function of dendritic cells

GAO Ying1, 2 |TAO Zhi-yong1 |XIA Hui 1* | YANG Wen-xuan1 |TAO Li 1 |FANG Qiang1 |MAI Yue-qin1   

  1. 1 Department of Microbiology and Parasitology|Bengbu Medical College|Anhui Province| Bengbu 233030|China;2 Department of Clinic Laboratory|Nanyang Central Hospital| Nanyang City| Henan Province| China
  • Online:2014-02-22 Published:2014-02-22
  • Contact: XIA Hui

PvMSP1对树突状细胞分化成熟和功能的影响

高迎1,2|陶志勇1|夏惠1*|杨文选1|陶莉1|方强1|买月琴1   

  1. 1 蚌埠医学院病原生物学教研室 (蚌埠 233030); 2 河南省南阳市中心医院
  • 通讯作者: 夏惠
  • 作者简介:高迎| 女| 硕士| 检验师。研究方向: 感染与免疫
  • 基金资助:
    安徽省教育厅自然科学研究重点项目 (KJ2012?A?200)

Abstract: Objective To investigate the effects of Plasmodium vivax merozoite surface protein 1(PvMSP1)on differentia? tion,maturation and function of dendritic cells(DC)and the mechanisms of PvMSP1 on the activation of DC via toll like receptors (TLR) . Methods DCs were incubated with different doses of PvMSP1(1.0,10.0,100.0 μg/ml)in vitro. The changes of CD83, CD86,and HLA?DR on DC were detected by flow cytometry(FCM);the expressions of cytokine IL?10 and IL?12 of DC were mea? sured by ELISA;the expressions of TLR4 and TLR9 mRNA of DC were measured by RT?PCR;the proliferation induction to autol? ogous lymphocytes of DC was measured by MTT. Meanwhile,the untreated DC and LPS inducing DC were as the negative control and positive control,respectively. All the data were analyzed statistically. Results Compared with the untreated DC,the propor? tions of CD83,CD86 and HLA?DR on DC induced by LPS and PvMSP1 increased significantly(all P < 0.05);the expressions of IL?10 and IL?12 of DC induced by LPS increased significantly(P < 0.01),and those induced by PvMSP1 also increased signifi? cantly(all P < 0.05) . In the LPS inducing group,the TLR4 mRNA production increased(P < 0.05)and the TLR9 mRNA produc? tion had no significantly changes(P > 0.05) . In the PvMSP1?treated group,the DC TLR4 mRNA production increased(P < 0.01) and the TLR9 mRNA production had no significantly changes(P > 0.05);DC stimulated the proliferation of autologous lympho? cytes. Conclusion PvMSP1 enhances DC differentiation and maturation,and the mature DC induced by PvMSP1 has the ability of antigen presenting. The route for PvMSP1 inducing DC maturation might be TLR4 pathway rather than TLR9 pathway.

Key words: Plasmodium vivax;PvMSP1; Dendritic cell(DC); TLR pathway;TLR4;TLR9; Differentiation

摘要: 目的 目的 观察间日疟原虫裂殖子主要蛋白1 (PvMSP1) 对树突状细胞 (DC) 分化成熟和功能的影响, 并探讨该蛋白通 过Toll样受体 (TLR) 通路活化DC的机制。方法 方法 选择不同剂量的PvMSP1 (1.0、 10.0、 100.0 μg/ml) 体外刺激人单核细胞来 源的DC, 采用流式细胞术分析DC成熟性相关分子CD83、 CD86、 HLA?DR的表达变化; ELISA检测DC培养上清中IL?10、 IL?12 的表达水平; RT?PCR检测DC TLR4、 TLR9 mRNA的表达水平; MTT法检测DC刺激自体淋巴细胞增殖的能力。同时选择未 刺激的DC作为阴性对照组, LPS刺激的DC作为阳性对照组。对所得数据进行方差分析和q检验。结果 结果 与未刺激组比 较, LPS诱导组CD83、 CD86、 HLA?DR的百分含量均增加, PvMSP1诱导组CD83、 CD86、 HLA?DR的表达也均升高 (P均 < 0.05); LPS诱导组IL?10、 IL?12的表达量明显增加 (P < 0.01), PvMSP1诱导组IL?10、 IL?12的表达量也均增加 (P均 < 0.05); LPS组DC TLR4 mRNA的表达增加 (P < 0.05), TLR9 mRNA的表达无明显变化 (P > 0.05), PvMSP1诱导组DC TLR4 mRNA 的表达增加 (P < 0.01), TLR9 mRNA无明显变化 (P > 0.05); DC能够刺激自体淋巴细胞增殖。结论 结论 PvMSP1具有促进DC 分化成熟的作用, 且经其诱导成熟的DC具备抗原递呈功能; PvMSP1可能经TLR4通路而非TLR9通路诱导DC成熟。

关键词: 间日疟原虫; 间日疟原虫裂殖子主要蛋白1 (PvMSP1); 树突状细胞 (DC); Toll样受体 (TLR) 通路; TLR4; TLR9; 分化

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