Abstract: Objective To explore the relationship between the lysozyme activities of Oncomelania hupensis snails among dif? ferent seasons，and the difference of the lysozyme activities among the different ages of the snails. Methods The homogenate soft tissues of O. hupensis were dissolved with Tris?HCl?TritonX?114 buffer and concentrated，and the enzyme was extracted by centrif? ugation. Results There were significant differences of lysozyme activities among different seasons（Spring，Summer，Autumn， Winter）with the results of 71.33±4.16 U/mg， 93.67±7.10 U/mg， 150.33±10.01 U/mg， 358±35.38 U/mg， respectively（F = 144.455， P ＜ 0.01） . There were also significant differences of the activities between two age groups（adults and young snails）with the re? sults of 93.67±7.10 U/mg and 69.33±5.03 U/mg， respectively（t = 4.845， P ＜ 0.05） . Conclusion There are significant differenc? es of the lysozyme activities among different seasons and ages of snails，and the highest lysozyme activity is in winter. The lyso? zyme activity of adult snails is higher than that of the youth.

Key words: Oncomelania hupensis； Lysozyme；Activity.

摘要： 目的 目的 探索湖北钉螺 （Oncomelania hupensis） 体内溶菌酶活力与季节变化的关系， 及不同螺龄钉螺体内溶菌酶活 力差异。方法 方法 采用钉螺软体组织匀浆， Tris?HCl?TritonX?114 缓冲溶液抽提， 经超滤浓缩获得粗制溶菌酶液， BCA法测定 蛋白质含量， 浊度法测定溶菌酶活力。分析不同季节、 不同螺龄钉螺体内溶菌酶活力差异。结果 结果 春、 夏、 秋、 冬季钉螺溶 菌酶活力分别为 （71.33±4.16）、（93.67±7.10）、（150.33±10.01）、（358±35.38）U/mg， 冬季活力最高， 差异有统计学意义 （F = 144.455， P ＜ 0.01）， 成螺和幼螺溶菌酶活力分别为 （93.67±7.10）U/mg和 （69.33±5.03）U/mg， 差异有统计学意义 （t = 4.845， P＜0.05）。结论 结论 湖北钉螺成螺体内溶菌酶活力随季节变化而改变， 成螺溶菌酶活力高于幼螺。

关键词: 湖北钉螺； 溶菌酶； 活力