Abstract: Objective To understand the pathological changes of tissues and organs in Microtus fortis infected with Schistoso? ma japonicum for further insight into the biological dynamic variation and explore immune mechanisms of M. fortis against S. japoni? cum infection. Methods The healthy M. fortis and mouse were infected with cercariae of S. japonicum through abdominal skin. On the day 12，20 and 40，the M. fortis and mouse were sacrificed by cervical dislocation and dissected. The liver and kidney slic? es of M. fortis and mouse of the different groups were prepared and stained with hematoxylin eosin stain（HE）by conventional tech? niques，respectively. Results We did not find obvious lesions on the internal organs of mouse 12 d and 20 d after the infection and the lesions of M. fortis 40 d after the infection. However，white nodules appeared on the liver，kidney and spleen of the M. for? tis 12 d and 20 d after the infection，and there were a lot of S. japonicum worms on pathological sections of inflamed liver and kid? ney tissues，and the boundary between worms and normal organization was clear. There were no parasites on pathological section of liver and kidney of mouse 12 d and 20 d after the infection. Conclusion Among M. fortis，there are severe immune responses and the immune responses have an individual difference 12 days after S. japonicum infection.

Key words: Schistosoma japonicum；Microtus fortis；Immune response；Pathological section

摘要：

目的 比较室内繁殖东方田鼠与昆明鼠感染日本血吸虫后不同阶段脏器病变以及体内日本血吸虫发育与存活状况。方法 采用日本血吸虫尾蚴感染健康东方田鼠和昆明鼠， 在感染后12、 20、 40 d剖杀， 观察并比较东方田鼠以及昆明鼠肝、 肾、 肺等脏器组织病变及体内日本血吸虫发育与存活情况。结果 日本血吸虫感染后12、 20 d昆明小鼠和感染40 d后东方田鼠的内脏器官均未出现明显病变； 感染12 d及20 d后的东方田鼠肝脏、 肾脏及脾脏出现明显白色结节， 且以感染12 d的组织病变更为明显， 部分东方田鼠病变组织仅见于肝脏。病理切片显示， 病变肝脏和肾脏组织中存在完整日本血吸虫虫体， 与正常组织界限分明； 而无病变的肝脏和肾脏组织病理切片未发现虫体。结论 室内繁殖东方田鼠感染日本血吸虫后12 d免疫反应剧烈且存在明显的个体差异。

关键词: 日本血吸虫； 东方田鼠； 免疫反应； 病理切片