Chin J Schisto Control ›› 2013, Vol. 25 ›› Issue (2): 141-.

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Dynamics of IL-22-producing cells of mice infected with Schistosoma japonicum

LI Yong|YANG Xiao-wei|DONG Xiao-xiao|CHEN Xiao- jun|XU Zhi -peng|ZHANG Wei -wei|XUE Xue|KONG Wen- jun| ZHOU Sha| ZHU Ji-feng|LIU Feng|SU Chuan*   

  1. Department of Pathogen Biology| Nanjing Medical University|Nanjing 210029| China
  • Online:2013-04-15 Published:2013-04-24
  • Contact: SU Chuan

小鼠感染日本血吸虫后IL?22来源细胞的动态观察

李永|杨晓玮|董潇潇|陈晓军|徐志鹏|张伟伟|薛雪|孔文珺|周莎|朱继峰|刘丰|苏川*   

  1. 南京医科大学病原生物系 (南京 210029)
  • 通讯作者: 苏川
  • 作者简介:李永| 男| 硕士研究生。研究方向: 感染免疫学
  • 基金资助:

    国家自然科学基金 (81271861); 江苏高校优势学科建设工程 (JX10131801016)

Abstract:

Objective To study the dynamics of IL?22?producing cells and analyze the main source of IL?22 of mice infected with Schistosoma japonicum. Methods The lymphocytes and splenocytes were prepared from lymphnodes and spleens of mice with S. japonicum infection at different stages(0 w,3 w,5w,8 w,13 w after infection) . The proportions of IL?22?producing cells were determined by FACS. Results The proportions of IL?22?producing cells increased significantly in the lymphocytes and sple? nocytes from the mice infected with S. japonicum. The IL?22?producing T cells accounted 80% of the total IL?22?producing cells. The IL?22?producing CD4- T cells increased obviously in the 3rd week after S. japonicum infection. However,the proportion of the IL?22?producing CD4+ T cells began to rise since the 3rd week post?infection,and increased significantly at the 8th week after in? fection. Conclusion In the lymphnodes and spleens of mice infected with S. japonicum,IL?22 is produced by both T cells and non?T cells,and T cells are the most important source of IL?22. The IL?22?producing CD4- T cells increase rapidly in the early acute inflammatory response, and the IL?22?producing CD4+ T cells increase significantly during the chronic inflammatory phase.

Key words: Schistosoma japonicum;Mouse; IL?22; T cell; CD4 cell

摘要:

目的 目的 观察C57BL/6小鼠感染日本血吸虫后, 体内细胞因子IL?22来源细胞的动态变化, 并分析小鼠体内IL?22的主要来源。方法 方法 分别提取感染日本血吸虫0、 3、 5、 8、 13周的小鼠淋巴结和脾脏细胞, 采用流式细胞术检测各个感染时期 IL?22来源细胞的比例。结果 结果 感染小鼠的淋巴结和脾脏中, 产生IL?22的细胞比例显著升高。在所有产生IL?22的细胞中, 有80%左右为T细胞。产生IL?22的CD4- T细胞的比例在感染后3周内明显升高, 而产生IL?22的CD4+ T细胞的比例到感染第3周后才开始升高, 至第8周显著增加。结论 结论 日本血吸虫感染小鼠的淋巴结和脾脏中, IL?22由T细胞和非T细胞两大群细胞产生, 其中T细胞是细胞因子IL?22最主要的来源。产生IL?22的T细胞分为CD4- T细胞和CD4+ T细胞两群, 其中产生IL?22的CD4- T细胞在日本血吸虫感染早期迅速增加; 而在感染进入慢性期后产生IL?22的CD4+ T细胞大量增加。

关键词: 日本血吸虫; 小鼠; IL?22; T细胞; CD4细胞

CLC Number: