Abstract:

suspension concentrate of 26% metaldehyde and niclosamide（MNSC）on Lymnaea. Methods WPN and MNSC were pre?
pared as a series of solutions containing the active ingredient concentrations of 0.06，0.13，0.25，0.50，1.00，2.00 mg/L and 4.00
mg/L，and the adult Lymnaea snails were soaked in the above mentioned series of solutions in the laboratory，and the LC50 values
were calculated. The doses of active ingredient concentrations of 0.03，0.06，0.13，0.25，0.50，1.00 g/m2 and 2.00 g/m2 of WPN
and MNSC were adopted to spray on Lymnaea snails in the laboratory，and the LC50 values were calculated. A series of solutions
containing the active ingredient concentrations of 1.00 mg/L，0.50 mg/L，0.25 mg/L，and 0.13 mg/L of WPN and MNSC were pre?
pared，and the adult Lymnaea snails were put into the bowls with each concentration solution above mentioned and the climbing
situation of the snails was observed at different time. Results By the immersion method，LC50 values of WPN at 48 h and 72 h
were 0.93 mg/L and 0.64 mg/L respectively；LC50 values of MNSC at 48 h and 72 h were 0.74 mg/L and 0.51 mg/L respectively；by
the spray method，when active ingredient concentrations of WPN and MNSC were 1.00 g/m2 or more，the death rates were both
100% after 3 days. In the climbing test，the Lymnaea snails did not climb in the solutions containing the active ingredient concen?
tration of 1.00 mg/L of WPN and MNSC，however，a few snails climbed in the low concentrations. Conclusions WPN and MNSC
both have the effect of killing Lymnaea snails and inhibiting their climbing. By using the immersion method in the field，the active
ingredient concentration of 2.00 mg/L of WPN and MNSC for 48 h is appropriate；by using the spray method，the active ingredient
content of 1.00 g/m2 of WPN and MNSC for 3 days is appropriate.

Key words: Wettable powder of 50% niclosamide ethanolamide salt（WPN）；Suspension concentrate of 26% metaldehyde and niclosamide（MNSC）；Lymnaea；Molluscicidal effect

摘要：

目的 观察50%氯硝柳胺乙醇胺盐可湿性粉剂（WPN）和26%四聚⋅杀螺胺悬浮剂（MNSC）杀灭椎实螺效果。方法将WPN和MNSC分别配制成有效成分浓度为0.06、0.13、0.25、0.50、1.00、2.00 mg/L和4.00 mg/L的溶液，在实验室浸泡椎实螺成螺，统计椎实螺死亡率并计算半数致死浓度（LC50值）。采用有效成分浓度分别为0.03、0.06、0.13、0.25、0.50、1.00g/m2和2.00 g/m2的WPN和MNSC开展室内喷洒杀灭椎实螺试验，统计椎实螺死亡率并计算计算LC50值。分别配制WPN和MNSC有效成分浓度为1.00、0.50、0.25、0.13 mg/L的溶液，分组投放椎实螺，观察不同时间椎实螺上爬情况。 结果 WPN浸杀48 h和72 h的LC50值分别为0.93 mg/L和0.64 mg/L，MNSC浸杀48 h和72 h的LC50值分别为0.74 mg/L和0.51 mg/L；WPN
和MNSC有效成分浓度> 1.00 g/m2时，喷洒3 d后椎实螺死亡率均达100%；在有效成分浓度为1.00 mg/L的WPN和MNSC药液中，未见椎实螺上爬，但在低浓度药液中有少量椎实螺上爬。结论 WPN 和MNSC均有杀灭椎实螺效果，且均可抑制椎实螺上爬。现场开展杀灭椎实螺工作时，浸杀法应采用WPN和MNSC有效成分浓度2.00 mg/L浸杀48 h以上为宜，喷洒法应采用有效成分含量1.00 g/m2喷洒3 d为宜。

关键词: 氯硝柳胺乙醇胺盐可湿性粉剂；四聚⋅杀螺胺悬浮剂；椎实螺；杀螺效果