Abstract:

Methods Rabbits were used for the animal model and Schistosoma japonicum eggs（1 mg/ml）were directly injected into
the brain by two ways of a bone drill or needle. The symptoms were observed and in the first and second week and later，the rab?
bits’brains were removed for pathological examinations. Results One to two weeks after the injection of schistosome eggs，the
rabbits had various neurological symptoms such as loss of appetite，hemiparesis，seizure，etc. The pathological analysis showed
the schistosome egg granuloma inflammatory reaction among 90% rabbits. Conclusion This new method of direct injection of S.
japonicum eggs through skull into the brain provides a good and easy animal model of neuroschistosomiasis.

Key words: Schistosomiasis；Neuroschistosomiasis；Experimental model；Schistosomal egg granuloma

摘要：

目的 建立一种新的家兔脑型血吸虫病模型以探讨脑型血吸虫病的形成机理及建模的影响因素。方法 以家兔为实验对象，分别通过钻孔法和锥颅法经颅直接注射日本血吸虫卵悬液1 ml（1 mg／ml）。观察临床症状，注射后第1周的第3、5天，第2周的第1、3、5天及2周后，取兔脑制作病理切片，观察其病理变化。结果 家兔在建模第1～2周即出现厌食、偏瘫、癫痫等多种神经系统症状。脑组织病理切片显示虫卵肉芽肿改变。建模成功率达90%。 结论 采用经颅直接注射虫卵法成功建立了脑型血吸虫病动物模型，为开展实验研究提供了基础。

关键词: 日本血吸虫病；脑型血吸虫病；动物模型；虫卵肉芽肿