Abstract:

Objective To evaluate the application effect of an ELISA kit for detection of IgG antibody to Schistosoma japoni?
cum in endemic areas. Methods In two schistosomiasis endemic villages of Eryuan County，Yunnan Province，there were 505
permanent residents enrolled in the study. Fecal examinations were carried out by using the nylon screen bag?egg concentration
method. In paralleled testing，the ELISA kit was used for the villagers to detect the IgG antibody to S. japonicum. The assay was
performed daily for 20 consecutive days，and the data were analyzed by comparison with the parasitological examination and by us?
ing the L?J quality control chart method. Results In 505 examinees，290 were positive by ELISA，with a positive rate of
57.43％. The L?J chart analysis showed that the deviation of A450 value between the quality control sera A and B was within the con?
trollable range. The fecal examination found 20 positives with a positive rate 3.96％. Compared with the fecal examination，the pos?
itive consistency rate of the ELISA method reached to 90.00%. Conclusion The ELISA kit for detection of IgG antibody to S. ja?
ponicum is stable，reliable and suitable for the screening of schistosomiasis in the field.

Key words: Schistosomiasis；ELISA；Quality control；Detection kit

摘要：

目的评价日本血吸虫IgG抗体检测试剂盒在血吸虫病流行区的应用效果。方法在云南省洱源县2个血吸虫病流行村选择505例常住居民作为检测对象，采用尼龙绢集卵孵化法进行血吸虫病病原法检测。采用Levey?Jennings（L?J）质控图法对整个试验过程进行质量控制。采用酶联免疫吸附试验（ELISA）对调查对象进行日本血吸虫IgG抗体检测，并对检测结果进行分析。结果505名检测对象中，ELISA法查出IgG抗体阳性者290例，阳性率为57.43％；质控血清A和质控血清B检测结果均在L?J质控图可控范围内，ELISA检测结果可信。尼龙绢集卵孵化法查出虫卵阳性者20例，阳性率3.96％。ELISA与粪检的阳性符合率为90.00％。结论日本血吸虫IgG抗体检测试剂盒有较好的稳定性、可靠性，适于现场血吸虫病人的筛查。

关键词: 血吸虫病；酶联免疫吸附试验；质量控制；试剂盒