Abstract:

Objective To predict and identify Taenia solium oncosphere TSO45?4B antigen FnⅢ structure domain linear B
cell epitopes. Methods The B cell epitopes of TSO45?4B FnⅢ structure domain were predicted through the sequence analysis by
using bioinformatics online tools and the protein space conformation was predicted by SWISS?MODEL. The peptides were synthe?
sized according to the predicted linear epitopes. The immunoreactivity of sera of cysticercosis patients to the peptides synthesized
was tested by using ELISA. Results Two linear B cell epitopes of TSO45?4B FnⅢ structure domain were predicted，and one of
the predicted epitope peptides synthesized could be recognized by the sera of cysticercosis patients. Conclusion Two linear B
cell epitopes of TSO45?4B FnⅢ structure domain are predicted and one of them has been confirmed successfully.

Key words: Taenia solium；Oncosphere；TSO45?4B；FnⅢ structure domain；B cell epitope

摘要：

目的预测并鉴定猪带绦虫六钩蚴TSO45?4B抗原FnⅢ结构域线性B细胞表位。方法利用生物信息学在线工具分析TSO45?4B抗原FnⅢ结构域序列并预测其线性B细胞表位；采用SWISS?MODEL软件预测TSO45?4B的蛋白空间结构，并合成两条预测表位肽。采用酶联免疫吸附试验（ELISA）检测猪囊尾蚴病人血清与两条预测表位肽的免疫反应性。结果获得2个TSO45?4B抗原FnⅢ结构域线性B细胞预测表位，其中1条预测表位合成肽可为猪囊尾蚴病患者血清识别。结论成功预测并鉴定了猪带绦虫六钩蚴TSO45?4B抗原FnⅢ结构域线性B细胞表位。

关键词: 猪带绦虫；六钩蚴；TSO45?4B；FnⅢ结构域；B细胞表位