Abstract:

Objective To develop a software that can be used to standardize optical density to normalize the procedures and
results of standardization in order to effectively solve several problems generated during standardization of in?direct ELISA results.
Methods The software was designed based on the I?STOD method with operation settings to solve the problems that one might en?
counter during the standardization. Matlab GUI was used as a tool for the development. The software was tested with the results of
the detection of sera of persons from schistosomiasis japonica endemic areas. Results I?STOD V1.0（WINDOWS XP/WIN 7，0.5
GB）was successfully developed to standardize optical density. A serial of serum samples from schistosomiasis japonica endemic ar?
eas were used to examine the operational effects of I?STOD V1.0 software. The results indicated that the software successfully over?
came several problems including reliability of standard curve，applicable scope of samples and determination of dilution for sam?
ples outside the scope，so that I?STOD was performed more conveniently and the results of standardization were more consistent.
Conclusion I?STOD V1.0 is a professional software based on I?STOD. It can be easily operated and can effectively standardize
the testing results of in?direct ELISA.

Key words: Schistosomiasis japonica；Indirect ELISA；Standardization；I?STOD V1.0 Software；I?STOD

摘要：

目的针对间接酶联免疫吸附试验（ELISA）检测日本血吸虫感染结果标准化中的几个常见问题，开发一款光密度值标准化软件，规范标准化过程和结果。方法以改良光密度值标准化变换方法（I?STOD）为开发依据，以Matlab GUI开发工具，综合I?STOD实施过程中的各个环节以及相应的处理方法进行软件开发，并用来自日本血吸虫病疫区的血清检测结果对该软件进行测试。结果成功开发了用于标准化光密度值的软件——I?STOD V1.0。该软件的运行环境为WIN?DOWS XP/WIN 7，所需硬盘容量约0.5 GB。用来自日本血吸虫病疫区的血清检测结果对该软件测试表明，该软件有针对性地解决了标准化过程中的标准曲线可靠性判断、样本适用范围及超范围样本稀释度确定等问题，使得I?STOD在应用上更简便、在操作上更规范。结论基于I?STOD方法开发的I?STOD V1.0应用软件操作简便，且能有效地对间接ELISA法的实验结果进行标准化。

关键词: 日本血吸虫病；间接ELISA；标准化；I?STOD V1.0软件；I?STOD