Chin J Schisto Control ›› 2012, Vol. 24 ›› Issue (5): 579-580.

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Infectivity difference between mice challenged by the laboratory ?cultivated and field collected Schistosoma japonicum?infected Oncomelania hupensis

  

  • Online:2012-10-11 Published:2012-10-11

日本血吸虫人工与自然感染性钉螺对小鼠的感染性

  

  1. 湖南省血吸虫病防治所、湖南省血吸虫病免疫与传播控制重点实验室、湖南省血吸虫病研究中心、湖南省血吸虫病诊断参比实验室(岳阳414000)
  • 作者简介:喻鑫玲|男|硕士|副主任技师。研究方向:血吸虫病免 疫学
  • 基金资助:

    “十一五”国家科技支撑计划资助(2009BAI78B07);湖南省卫生厅科研基金项目(132008?058)

Abstract:

Objective To explore the infectivity difference between the mice challenged by laboratory?cultivated and field collected Schistosoma japonicum?infected Oncomelania hupensis snails. Methods A total of 120 Kunming mice were randomly di? vided into two groups. S. japonicum?infected O. hupensis releasing cercariae was according to routine cercaria shedding method. Each mouse was challenged by 40 cercariae. The worm?load,the development rate,EPG of liver and EPG of feces in the mice were calculated. Results The mean worm?load,adult worm development rate,EPG of liver and EPG of feces in the group of mice infected by field collected S. japonicum?infected snails were 27.43±3.78,68.53±9.44,19 800.97±6 752.59 and 196.37±11.56,re? spectively,which were significantly higher than those in the group challenged by cercariae from laboratory?cultivated S. japonicum? infected snails(23.93±4.93,59.83±12.32,5 803.69±1 560.49 and 107.73±10.32)(P < 0.05). The mean worm?load,adult worm development rate and EPG of liver in the male mouse group were higher than those in the female mouse group(P < 0.05). Conclu? sion The cercariae released from field collected S. japonicum?infected snails have more aggressive infection ability,compared with the cercariae released from the laboratory?cultivated S. japonicum?infected snails and the results also show male mice are more susceptibility to the schistosome infection than female mice.

Key words: Oncomelania hupensis;Schistosome ;Cercaria;Infectivity, Mouse

摘要:

目的探讨人工培育与自然感染的日本血吸虫感染性钉螺对小鼠感染性的差异。方法将120只昆明种小鼠随机分为2组,将人工与自然感染性钉螺用常规法逸蚴,每只小鼠感染40条尾蚴,以平均虫荷、成虫发育率、每克肝脏虫卵数(肝卵EPG)和每克粪便虫卵数(粪卵EPG)为指标进行观察,并对观察结果进行统计分析。结果自然感染性钉螺感染小鼠的平均虫荷、成虫发育率、肝卵EPG及粪卵EPG分别为27.43±3.78、68.53±9.44、19 800.97±6 752.59、196.37±11.56,均明显高于人工组的23.93±4.93、59.83±12.32、5 803.69±1 560.49、107.73±10.32(P均< 0.05);相同数量的尾蚴感染后,雄性组小鼠的虫荷、发育率、肝卵EPG均高于雌性组(P均< 0.05)。结论自然感染性钉螺对小鼠的感染性明显高于人工感染性钉螺,且雄性小鼠比雌性小鼠更易感染血吸虫尾蚴。

关键词: 日本血吸虫;钉螺;尾蚴;感染性;小鼠

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