Abstract:

Objective To compare the effects of collagen fiber staining between Van?Gieson staining and Masson trichrome staining of hepatic specimens in mice with Schistosoma japonicum infection. Methods A model of hepatic granuloma and fibrosis was established by infecting mice with S. japonicum cercariae，then the hepatic specimens were taken and Van?Gieson staining and Masson trichrome staining were performed. Eventually，the area of granuloma and fibrosis were measured by imaging analysis software. Results When the time of staining was 3-7 min，there was no significant difference of the fibrosis areas between the two methods（P > 0.05）；when the time of staining was more than 10 min，the staining area showed by Masson?? s staining was sig? nificantly larger than that showed by Van?Gieson staining，and the difference was statistically significant（P < 0.05） . Conclu? sion The operation procedures of Van?Gieson staining are simpler and easier to master than those of Masson trichrome staining， therefore Van?Gieson staining is a better method to display collagen.

Key words: Schistosoma japonicum；Hepatic fibrosis；Van?Gieson staining；Masson staining

摘要：

目的 比较Van?Gieson氏染色和Masson氏三色染色法用于日本血吸虫感染小鼠肝脏纤维化染色的效果。 方法 建立日本血吸虫感染小鼠肝脏组织纤维化模型， 取肝脏标本， 常规切片， 进行Van?Gieson氏染色和Masson氏三色染色， 应用图像分析软件统计虫卵肉芽肿纤维化面积。 结果 染色时间为3～7 min时， 2种方法显示肉芽肿纤维化面积差异无统计学意义 （P＞0.05）； 染色时间为10 min时， 由于非特异性因素使得Masson氏染色法着色面积明显大于Van?Gieson氏染色法， 两者差异有统计学意义 （P＜0.05）。 结论 Van?Gieson氏染色法比Masson氏三色操作过程相对简单， 易于掌握，染色结果稳定、 可靠， 可以作为评价日本血吸虫感染小鼠肝脏纤维化程度的较好染色方法。

关键词: 日本血吸虫； 肝脏纤维化； Van?Gieson氏染色； Masson染色