Abstract:

Objective To investigate the dynamic expressions of IL?17 and IL?23 in mice with Schistosoma japonicum infec? tion. Method A murine model of the infection of S. japonicum was established. The suspension of spleen cells incubated with Co? nA was collected at 0，1，2，4，6，8 and 10 weeks post?infection. Sandwich ELISA and RT?PCR were used to measure the expres? sions of IL?17A and IL?23p19 on protein and mRNA level. Results The dynamic changes of IL?17A and IL?23p19 showed a posi? tive correlation. The level of IL?17A increased and reached the peak at 1 week after the infection，reduced at 4 weeks after the in? fection，and was even lower at 8 weeks post?infection. The level of IL?17 mRNA increased at 1 week post?infection，and then de? creased gradually at 2 weeks post?infection. Being consistent with the dynamic expression of IL?17A，the IL?23p19 expression in? creased at 1 week post?infection，went up to the peak at 2 weeks post?infection，and gradually reduced into the normal level at 4 weeks. However，the expression of IL?23p19 mRNA fluctuated in the normal range，increased at 4 weeks post ? infection，and reached the peak at 6 weeks post?infection. Conclusions IL?17 and IL?23 are of co?expression in the mice after schistosome in? fection. There is a significant increase in the early stage of the infection. IL?17 and IL?23 may play important roles during the im? mune process in the very early stage of Schistosoma infection.

Key words: Schistosoma japonicum；IL?17；IL?23；Immune reaction； Mouse

摘要：

目的 观察日本血吸虫感染小鼠IL?17A、 IL?23p19的表达， 探讨IL?17、 IL?23在免疫调控中的作用。 方法 建立日本血吸虫感染的小鼠模型， 采用ELISA和RT?PCR法检测小鼠感染后1、 2、 4、 6、 8及10周脾细胞培养液上清中IL?17A、 IL? 23p19蛋白水平和mRNA水平的表达。 结果 蛋白及mRNA表达结果显示， 感染小鼠IL?17A与IL?23p19的变化趋势基本一致。感染小鼠IL?17A蛋白表达水平于感染后1周迅速升高， 之后持续下降， 8周时表达水平低于正常对照组； 其mRNA 表达也于感染后1周升高， 第2周开始下降。而IL?23p19蛋白的表达水平于感染后1周升高， 第2周到达高峰， 第4周开始下降； 其mRNA水平起先一直在正常范围波动， 感染后4周开始升高， 第6周达到峰值， 之后逐渐下降。 结论 IL?17和IL? 23在日本血吸虫感染小鼠体内协同表达， 并于感染早期呈现显著上升趋势， 提示IL?17、 IL?23参与了血吸虫感染极早期的免疫病理过程。

关键词: 日本血吸虫； IL?17； IL?23； 免疫反应； 小鼠