Chin J Schisto Control ›› 2011, Vol. 23 ›› Issue (4): 424-427.

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Effects of male worm extraction on ultrastructure of cultured vitelline cells from Schistosoma japonicum

Zhong Qin-ping| Li Jun-lin|Ming Zhen-ping| Jiang Ming-sen| Dong Hui-fen*   

  1. Department of Medical Parasitology and Research Laboratory of Schistosomiasis|School of Basic Medical Science|Wuhan University|Wuhan 430071|China
  • Online:2011-08-15 Published:2011-08-05
  • Contact: Dong Hui-fen

日本血吸虫雄虫抽提物对卵黄培养细胞超微结构的影响

钟沁萍|李俊琳|明珍平|蒋明森|董惠芬*   

  1. 武汉大学基础医学院人体寄生虫学教研室、血吸虫病研究室(武汉|430071)
  • 通讯作者: 董惠芬
  • 作者简介:钟沁萍|女|硕士|实验师。研究方向:血吸虫细胞生物学
  • 基金资助:

    国家自然科学基金(30570243)

Abstract:

Objective To study the effects of male worm extraction on the proliferation and metabolic activity of cultured vitelline cells from Schistosoma japonicum. Methods The 28-days adult S. japonicum were harvested by perfusion. The male and female of them were isolated after asepsis separately. The vitelline glands of female worms were isolated, and the vitelline cells were harvested by the cold digestion, then they were inoculated with the moist system method on the walls of culture flasks. The cultured vitelline cells were randomly divided into test and control groups. The cells in the control group were cultured in routine media and those in the test group were cultured in routine media containing male worm extraction of the concentration of 100 μg/ml. When cultured for 7 days, the cells in both group were prepared for observation under transmission electron microscope. Results In the test group, the numbers of mature vitelline cells were more than that in the control group; the cytoplasm and nucleus of mature vitelline cells were homogeneous stain. The nucleolus and rough-surfaced endoplasm reticula were clear, the intervals of vitelline globules were clear and their numbers could be counted. The number of mitochondria was small and the electron density was low; abundant rough-surfaced endoplasm reticula was found in the immature vitelline cells. In the control group, there were more immature vitelline cells than in the test group. The cytoplasm of the cultured vitelline cells took changes of balloon, especially in mature vitelline cells, vitelline globules fused each other, no mitochondria was found; in immature vitelline cells, the space between vitelline globules and the membrane surrounding them broadened gradually and vitelline globules were released and uncovered; rough-surfaced endoplasmic reticula enlarged, space vacuolated and the ribosomes dropped; and the number of lipid increased. Conclusions The male worm extraction can stimulate the development and survival of the cultured vitelline cells.

Key words: Schistosoma japonicum, Cultured vitelline cell, Male worm extraction, Ultrastructure

摘要:

目的 观察日本血吸虫雄虫抽提物对卵黄培养细胞超微结构的影响。方法 灌注法获取28 d虫龄的日本血吸虫虫体,无菌处理后分离雌雄虫体。取雌虫卵黄腺段虫体冷消化法制备细胞悬液,将细胞接种于培养瓶中,随机分对照组和实验组。对照组以常规培养基培养,实验组以常规培养基含100 μg/ml雄虫抽提物培养。培养第7天,取实验组与对照组细胞制备标本并观察。结果 实验组成熟卵黄细胞出现机率较对照组高,核和胞质染色均匀;卵黄滴内的卵黄球之间界限清晰,数目可数;线粒体数目较少,电子密度较低。未成熟卵黄细胞胞质中粗面内质网较丰富。与实验组相比,对照组中卵黄细胞的核和胞质电子密度降低,脂滴数目增多,空泡化程度严重,未见线粒体;成熟卵黄细胞中卵黄滴内的卵黄球之间已发生融合,有卵黄球从中释放出来,成为裸露体;未成熟卵黄细胞中卵黄球与外面包绕的膜之间空隙增大,粗面内质网扩大和囊泡变,核糖体脱颗粒。结论 日本血吸虫雄虫抽提物对雌虫卵黄细胞在体外的发育与存活具有促进作用。

关键词: 日本血吸虫, 卵黄培养细胞, 雄虫抽提物, 超微结构

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