Chin J Schisto Control ›› 2010, Vol. 22 ›› Issue (1): 17-19.

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Effect of Kupffer cells on CD4+CD25+ T cells in schistosome granuloma

Yang Jiang-hua1, He Lei2, Wang Tao1, Yang Jin-sun1, Hou Wei-shun1, Su Chuan2   

  1. 1 Department of Infectious Diseases, Yijishan Hospital affiliated to Wannan Medical College, Wuhu 241001, China|2 Department of Pathogenic Biology, Nanjing Medical University, China
  • Online:2010-02-15 Published:2010-03-10

Kupffer 细胞对日本血吸虫病肉芽肿期CD4+CD25+T 细胞的影响

杨江华1|贺蕾2|王涛1|杨进孙1|侯为顺1|苏川2   

  1. 1 皖南医学院附属弋矶山医院感染性疾病科（芜湖 241001）；南京医科大学病原生物学系
  • 作者简介:杨江华|男|博士|副主任医师。研究方向：病原体慢性感染的免疫机制
  • 基金资助:

    安徽省自然科学基金（070413111）；国家自然科学基金青年基金（3070694）

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Abstract:

[Abstract] ObjectiveTo explore the effect of targeting Kupffer cells on CD4+CD25+ T cells in schistosomiasis granuloma. Methods Six to eight-week-old C57BL/6 female mice were divided into three groups, a control group, an infection group with S. japonicum cercariae and an infection group injected with GdCl3 through the penile vein (15 mg/kg) twice per week. After 8 weeks of the infection, the number of CD4+CD25+Tcells were detected by using flow cytometry and the number of Foxp3 was detected by using immunohistochemistry.For the detection of murine IL-4, IL-5, IL-10, TGF-β1 and IFN-γ, a DuoSet ELISA development kit was used.Results The number of CD4+CD25+Tcells and the level of IL-10 decreased in the infection group injected with GdCl3 comparing with the infection group. GdCl3treatmentdecreased Foxp3 production and the level of ALT, and reduced the inflammatory response in schistosomiasis granuloma. Conclusion Kupffer cells can regulate the response of CD4+CD25+ T cells in schistosomiasis granuloma.

Key words: Schistosoma japonicum, CD4+CD25+ T cell, Kupffer cell

摘要：

[摘要] 目的 探讨Kupffer细胞对日本血吸虫病肉芽肿期CD4+CD25+T细胞的影响。方法 6～8周龄雌性C57BL/6J小鼠分为对照组，感染组与感染/氯化钆组3组，每组各多少只，是否在分组之前就感染了尾蚴？其中感染组怎么处理；感染/氯化钆组几天之后注射经尾静脉注射氯化钆，剂量为每次15 mg/kg，每周2次；对照组怎么处理？感染8周后流式细胞仪检测CD4+CD25+T细胞数量；免疫组织化学染色检测Foxp3的分布；酶联免疫吸附试验检测血清细胞因子IL-4、IL-5、IL-10、TGF-β1与 IFN-γ的水平，并进行肝功能检测。结果 日本血吸虫感染组小鼠CD4+CD25+T细胞数量为13.8％，感染/氯化钆组为9.3%（P<0.05）；感染组IL-10为41.4  pg/ml，感染/氯化钆组为22.6 pg/ml；氯化钆可下调Foxp3的分布、血清丙氨酸氨基转移酶的水平以及减轻血吸虫肉芽肿周围的炎症反应。结论 Kupffer细胞通过调控CD4+CD25+T细胞参与日本血吸虫肉芽肿的形成。

关键词: 日本血吸虫, CD4+CD25+T 细胞, Kupffer 细胞

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