Abstract:

 Objective   To explore the feasibility of detection of Schistosmna jaPonicum antibodies
in the sera of patients with schistosomiasis japonica (Sj-S) by Dot-ELISA with HRP-ASSP obtained
by labelling Ascaris suum soluble protein (ASSP) with horseradish peroxidase.  Methods   The reac-
tion of HRP-ASSP which repowed by labelling ASSP with HRP in accordance with the improved so-
dium periodate method to the sera of rabbits immunized with ASSP was observed by Dot-ELISA, and
its titer was detected.  Ten samples of sera of patients with schistosomiasis japonica were detected by
direct Dot-ELISA and indirect Dot-ELISA with HRP-ASSP and compared with 10 samples of sera of
healthy persons (Hp-S) ,  8 samples of sera of patients with paragonimiasis (P-S) ,  10 samples of sera
of patients with cysticercosis (C-S) and 6 samples of sera of patients with trichinelliasis (T-S). Re-
sults  It was positive that HRP-ASSP reacted with the sera of rabbits immunized with ASSP. The
positive reaction rate was 100% in detecting Sj-S by direct Dot-ELISA and indirect Dot-ELISA
However, the positive reaction rate was from 20% t0 50% in detecting Hp-S, P-S, C-S and T-S by
direct Dot-ELISA, and it was O in detecting these sera by indirect Dot-ELISA  Condusions   There is
a high affinity between ASSP and the antibodies of Schistosmna jaPonicum ,  and the ASSP is a poten-
tial diagnoslic antigen for detection of the antibodies of Schistosuma jaPonicum in schistosome infec-
ted people's sermn

Key words:  Ascaris suum ; , Soluble protein; , Schistosoma japonicum, Dot-ELISA

摘要：

目的探讨辣根过氧化物酶标记猪蛔虫可溶性蛋白质( HRP-ASSP)用于Dot-ELISA检测日本血吸虫病患者血清(Sj-S)中抗日本血吸虫抗体的可行性。方法参照改良过碘酸钠法，采用HRP标记ASSP得到HRP-ASSP，经Dot-ELISA观察HRP-ASSP与ASSP免疫兔血清反应，并测定效价；再将HRP-ASSP分别用于直接Dot-ELISA和间接Dot-ELISA检测10份Sj-S，并与检测10份健康人血清(Hp-S)、8份卫氏并殖吸虫病患者血清(P-S)、10份囊尾蚴病患者血清(C-S)和6份旋毛虫病患血清(T-S)进行比较。结果  HRP-ASSP与ASSP免疫兔血清呈阳性反应；直接Dot-ELISA检测Sj-S阳性反应率为100%，Hp-S、P-S、C-S、T-S阳性反应率为20%一50%；间接Dot-ELISA检测Sj-S阳性反应率为100%，其他血清阳性反应率均为O。结论ASSP与日本血吸虫特异性抗体可能具有高亲和力，HRP-ASSP用于间接Dot-ELISA检测Sj-S中抗日本血吸虫抗体具有潜在的应用价值。

关键词:  猪蛔虫；可溶性蛋白质；日本血吸虫；Dot-ELISA