Chin J Schisto Control ›› 2007, Vol. 19 ›› Issue (3): 196-199.

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Effects of culture supernatant of Toxoplasma gondii on CD4+CD25+ T cells in mice in vitro

 Zhang Gai, Ge Yi-yue, Wu Jiang-ping, Wang Yong, Hu Wei, Tan Ming-juan    

  • Online:2013-01-15 Published:2013-01-21

弓形虫培养上清体外对CD4+CD25+T细胞的影响

张改1|葛以跃1|吴江平2|王勇H|胡伟1|谭明娟1   

  1. 1南京医科大学病原生物学系(南京210029)|2江苏省南京市妇幼保健院
  • 作者简介:张改(1980-)|女|硕士研究生。研究方向:寄生虫分子免疫学

Abstract:

 Objective  To explore the effect of culture supernatant of Toxoplasma gondii on
CD4+CD25+ T cells in nuce in vitro.  Methods   CD4+CD25+ T cells were separated from spleens of
C57BL/6 mice and incubated in the culture superntant of Toxoplasma gondii. The apoptosis per-
centage of the CD4+CD25+ T cells were detected by FACScan, and the suppression of the CD4+
CD25+ T regulatory cells were examined by 3H_TdR incorporation. Results  Compared with the
CD4+CD25+ T cells incubated with RPMI-1640,  (36. 90±0. 36)% CD4+CD25+ T cells took apop-
tosis after incubated with the culture supernatant of Toxoplasma gondii for 10 hours,and the An-
 nexin-v positive rate increased by (13. 60士2. 15) %.  Compared with RPMI-1640, the culture super-
natant of Toxoplasma gondii incubating the CD4+CD25+ T regulatory cells for 5,10 hours reduced
 their suppressive potential on the proliferation of the CD4+CD25- T cells significantly.  Conclusions
  Some composition of the culture supernatant of Toxoplasma gondii might cause apoptosis of
CD4+CD25+ T cells and as a result, could reduce their suppression on CD4+CD25- T cells.

Key words: Toxoplasma gondii, CD4+CD25+ T cell, Suppression, Apoptosis

摘要:

目的探讨体外培养条件下弓形虫培养上清对CD4+CD25+T细胞的影响。方法用弓形虫培养上清和自小鼠脾脏分离的CD4+CD25+T细胞共同孵育,Annexin-v染色法检测CD4+CD25+T细胞的凋亡情况,淋巴细胞增殖实验检测CD4+CD25+T细胞对CD4+CD25-T细胞的抑制功能。结果  用弓形虫培养上清孵育10 h的小鼠脾脏CD4+CD25+T细胞有(36. 90±0.36)%出现凋亡,和RPMI-1640孵育组相比,凋亡率上升了(13. 60士2.15)%。与RPMI-1640孵育组相比,用弓形虫培养上清处理5、10 h的小鼠脾脏CD4+CD2 5+T细胞对CD4+CD25 -T细胞的抑制功能下降(P<0.01)。结论  弓形虫培养上清中可能存在某些成分能够诱导CD4+CD25+T细胞出现凋亡,并使CD4+CD25+T缅胞对CD4+CD25-T细胞的抑制功能下降。

关键词: 弓形虫;CD4+CD25+T细胞;抑制功能;凋亡

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