Chin J Schisto Control ›› 2007, Vol. 19 ›› Issue (05): 374-376.

Previous Articles     Next Articles

Morphological observation on the reproductive system of adult Schistosoma japonicum under an optical microscope

 Wang Xiao-nan, Wu 2heng-sheng, Yang Feng, Shen Ji-jia   

  • Online:2012-12-26 Published:2013-01-05

光学显微镜下日本血吸虫成虫生殖系统形态学观察

王晓楠|吴正升|杨枫|沈际佳   

  1. 1 |安徽医科大学病原生物学教研室(合肥230032);2  安徽医科大学病理学教研室
  • 作者简介:王晓楠(19 82 - )|女|硕士研究生。研究方向:血吸虫病分子生物学与分子疫苗

Abstract:

 Objective  To investigate the morphological features of reproductive system of adult
Schistosoma japonicum under an optical microscope. Methods  Adult schistosomes were obtained
from infected mice with cercariae shedding from Oncomelania snails. The adult worms fixed with
10% formalin, dehydrated, imbedded in paraffin, cut at 3 rim thick, stained by HE staining and
then observed under an optical microscope. Results   The reproductive organs of adult Schistosoma
japonicum such as testicle, ovary, fallopian tube, vitellarium, yolk duct and hystera were displayed
distinctly and typically. Conclusions  The morphological features of reproductive system of adult
Schistosoma japonicum are distinct and typical by using routine pathological techniques preparing
and HE staining, which establishes a morphological foundation for the morphological teaching of
schistosomes and reproductive biology research.

Key words: Schistosoma japonicum, Adult worm, Reproductive system, Optical microscope, Morphology

摘要:

目的在光学显微镜下观察日本血吸虫雌雄成虫生殖系统的组织形态结构特征。方法感染性钉螺逸出尾蚴,经腹部皮肤感染小鼠,感染7周后解剖获得血吸虫成虫,经10%甲醛固定、脱水、石蜡包埋、切片和苏木素伊红(HE)染色后在光镜下观察。结果光镜下日本血吸虫雌雄成虫睾丸、卵巢、输卵管、卵黄腺、卵黄管、子宫等生殖器官的组织结构和细胞形态清晰。结论  日本血吸虫成虫经石蜡切片和HE染色后,雌雄成虫各生殖器官在光镜下清晰可见,为血吸虫形态学教学和血吸虫生殖生物学研究提供了简便的方法。

关键词: 日本血吸虫;成虫;生殖系统;光学显微镜;形态学

CLC Number: