Abstract: Objective Objective To analyze the enzyme kinetics of active ingredient of Buddleja lindleyana （AIBL）against Oncomela? nia hupensis，the intermediate host of Schistosoma japonicum. Methods Methods O. hupensis snails were placed in 1 000 ml of 3.55 mg/L AIBL solution for 24，48 h and 72 h，respectively，and the enzyme kinetics of alanine aminotransferase（GPT）was deter? mined by Reitman?Frankel assay，lactate dehydrogenase（LDH）by the chemical inhibition lactic acid substrate method，alka? line phosphatase（AKP）by the disodium phenyl phosphate colorimetric method，acetylcholine esterase（AChE）and malate de? hydrogenas（MDH）by ELISA，and succinate dehydrogenase（SDH）by the phenazine methyl sulfate reaction method（PMS）in the soft tissues of O. hupensis before and after AIBL treatment. Results Results Following exposure to 3.55 mg/L AIBL solution for 24 h， the GPT，LDH，and AKP activities significantly improved in the soft tissues of O. hupensis，while the SDH and MDH activities were significantly lowered in the head?foot and liver. However，AIBL treatment did not cause significant effect on AChE activity in O. hupensis. Conclusions Conclusions AIBL causes significant damages to O. hupensis liver and can efficiently act on anaerobic and aer? obic respiration loci，which will hinder energy metabolism，and cause inadequate energy supply in cells used for normal secre? tion， eventually leading to O. hupensis death.

Key words: Oncomelania hupensis；Buddleja lindleyana；Enzyme kinetics；AIBL

摘要： 目的 目的 分析醉鱼草有效组分 （AIBL） 对湖北钉螺的酶促动力学影响。方法 方法 湖北钉螺浸泡于3.55 mg/L AIBL溶 液中， 分别于浸泡后24、 48、 72 h时用赖氏法测定钉螺GPT活性， 用化学抑制乳酸底物法测定LDH活性， 用磷酸苯二钠比 色法测定AKP活性， ELISA法测定AChE和MDH活性， 硫酸甲酯吩嗪反应法测定钉螺SDH活性。结果 结果 浓度为3.55 mg/L 的AIBL溶液在浸泡湖北钉螺24 h后， 钉螺GPT、 LDH、 AKP 活性显著提高， 钉螺头足部及肝脏SDH和MDH活性显著降 低， 但对AChE活力无影响。结论 结论 AIBL 能显著损害湖北钉螺肝脏、 作用于无氧和有氧呼吸作用位点、 影响能量代谢， 并 导致能源供应不足， 最终导致钉螺死亡。

关键词: 湖北钉螺； 醉鱼草； 酶促动力学； AIBL