Chin J Schisto Control ›› 2015, Vol. 27 ›› Issue (5): 549-.DOI: 10.16250/j.32.1374.2015112

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Quality analysis of malaria blood smear preparations of febrile patients in Chenzhou Prefecture, 2014

WANG Yan-qin1 | ZHU Han-wu2*   

  1. 1 Department of Preventive Medicine and Medical Examination|Xiangnan University|Chenzhou 423000|China;2 Chenzhou Center for Disease Control and Prevention|Hunan Province|China
  • Online:2015-10-21 Published:2015-10-21

2014年郴州市不明原因发热病人疟原虫血片制作质量分析

王艳琴1|朱韩武2*   

  1. 1 湘南学院预防医学与医学检验系 (郴州 423000); 2 湖南省郴州市疾病预防控制中心
  • 通讯作者: 朱韩武
  • 作者简介:王艳琴| 女| 助教。研究方向: 临床检验
  • 基金资助:
    湘南学院科研项目 (2014XJ57); 湖南省 “十二五” 重点建设学科项目 (湘教发 ﹝2011﹞ 76号); 湘南学院 “十二五” 重点建设学科项目 (湘南学院院发 ﹝2012﹞ 26号)

Abstract: Objective Objective To standardize the malaria smear preparations of febrile patients in Chenzhou Prefecture,Hunan Province,so as to provide the technical support for malaria elimination. Methods Methods According to the Technical Solutions to Eliminate Malaria(2011 edition),the blood smear preparations of febrile patients from each county,included more than 3% negatives and all positives,were reviewed monthly in 2014. The quality of blood smear manufacture,dyeing,cleanliness and re? sults was reviewed by malaria microscopic examination experts. The data were analyzed with the descriptive epidemiological methods. Results Results Totally 231 blood smears were reviewed in 2014 with a reviewed rate of 6.91%. The blood smear production qualified rate was 80.52%,the dyeing pass rate was 84.42%,the cleanliness pass rate was 86.58%,and there were no false de? tections and no leak detections. The highest blood smear production qualified rate,dyeing pass rate and cleanliness pass rate were found in Guiyang County and Linwu County,with all the rates of 100% respectively. The lowest blood smear production qualified rate and cleanliness pass rate were found in Yizhang County,with the rates of 52.94 % and 70.59% respectively. The lowest blood smear dyeing pass rate was found in Yongxing County with the rate of 63.64%. There were statistically significant differences between Guiyang County and Yizhang County in the production qualified rates and cleanliness pass rates( χ2 = 18.60,9.73,both P < 0.01) . There was a statistically significant difference between Guiyang County and Yongxing County in the dyeing pass rates(χ2 = 11.43,P < 0.01) . Conclusion Conclusion Through the review of blood smears,the problems will be timely discovered. Therefore, the blood smear quality should improve, which is helpful for achieving the goal of malaria elimination.

Key words: Plasmodium;Microscopy;Fever;Blood smear;Quality;Chenzhou Prefecture

摘要: 目的 目的 规范郴州市不明原因发热病人疟原虫血片制作质量, 为消除疟疾提供技术保障。方法 方法 按 《消除疟疾技 术方案 (2011年版)》 要求, 在2014年每月抽取郴州市各县 (市、 区) 不明原因发热病人疟原虫镜检血片, 其中阴性血片抽 取不少于3%, 阳性血片全部抽取, 疟疾镜检专家对抽取血片的制作、 染色、 清洁度和血检结果进行复核, 并采用描述性流 行病学方法对血片复核数据进行统计分析。结果 结果 2014年, 全市共复核血片231张, 复核率为6.91%, 血片制作合格率为 80.52%, 血片染色合格率为84.42%, 血片清洁度合格率为86.58%, 未发现误检和漏检。血片制作合格率、 染色合格率和 清洁度合格率最高的是桂阳县和临武县 (均为100.00%), 血片制作合格率和清洁度合格率最低的是宜章县 (分别为 52.94%、 70.59%), 血片染色合格率最低是永兴县 (63.64%); 桂阳县与宜章县血片制作合格率和清洁度合格率差异有统 计学意义 ( χ2 制作 = 18.60,χ2 清洁度 = 9.73, P均< 0.01), 桂阳县与永兴县血片染色合格率差异有统计学意义 (χ2 = 11.43, P < 0.01)。结论 结论 通过抽查复核血片质量, 及时发现血片质量问题, 可促进血片质量提升, 有助于消除疟疾目标的实现。

关键词: 疟原虫; 镜检; 发热; 血片; 质量; 郴州市

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