Chin J Schisto Control ›› 2015, Vol. 27 ›› Issue (6): 612-.DOI: 10.16250/j.32.1374.2015110

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Research on dust mite allergen gathered from filters of air-conditioners

ZHAN Xiao-dong1| WU Hua2| HU Hui-min1| LI Chao-pin1*   

  1. 1 Department of Medical Parasitology| Wannan Medical University| Wuhu 241002| China;2 The 2nd Affiliated Hospital| Wannan Medical University| Anhui Province| China
  • Online:2015-12-22 Published:2015-12-22
  • Contact: LI Chao?pin

空调器隔尘网富集尘螨过敏原的研究

湛孝东1|吴华2|胡慧敏1|李朝品1*   

  1. 1 皖南医学院医学寄生虫学教研室 (芜湖 241002); 2皖南医学院第二附属医院
  • 通讯作者: 李朝品
  • 作者简介:湛孝东| 男| 博士研究生| 讲师。研究方向: 螨类与变态反应性疾病研究
  • 基金资助:
    国家自然科学基金 (30872367); 安徽省教育厅自然科学研究项目 (2006kj101A; KJ2014A270); 皖南医学院重点科研项目培育基金 (WK2013Z05)

Abstract: Objective To discuss the relation between the dust mite allergen (Der) in air?conditioner filters and the asthma attack. Methods The dust samples were collected from the filters of air?conditioners in dining rooms, shopping malls, hotels and households, respectively. The concentrations of Der f 1 and Der p1 were detected by ELISA, and the dust mite immune activities were determined by dot?ELISA. Results The concentrations of Der f 1 in the dining rooms, shopping malls, hotels and households were 1.52, 1.24, 1.31 μg/g and 1.46 μg/g respectively, and the concentrations of Der p 1 were 1.23, 1.12, 1.16 μg/g and 1.18 μg/g, respectively. One hour after the running of air?conditioners, the concentrations of Der f 1 and Der p 1 in the air were higher than those before the running of air?conditioners, and the differences were significant (P < 0.05). Additionally, the dot?ELISA results revealed that the allergen extracted from the dust was capable of reacting with IgE from the sera of asthma mice allergic to dust mites. Conclusion Der f 1 and Der p 1 appear abundantly in the filters of air?conditioners in domestic houses in Wuhu City, and the allergens can induce asthma.

Key words: Dust mite; Allergen; Air?conditioner filter

摘要: 目的 探讨空调隔尘网中尘螨过敏原与哮喘发病的关系。方法 分别从芜湖市区食堂、商场、酒店和居民家中空调器隔尘网上,及其附近空气中采集灰尘样本各10份。用ELISA法检测粉尘螨1类过敏原(Der f 1)、屋尘螨1类过敏原(Der p 1)的浓度及其灰尘提取液过敏原性。结果 芜湖市区食堂、商场、酒店和居民家中空调器隔尘网灰尘中的过敏原Der f 1浓度分别为1.52、1.24、1.31 μg/g 和1.46 μg/g ;Der p 1浓度分别约为1.23、1.12、1.16 μg/g 和1.18 μg/g;2种过敏原在开机1 h后的空气中浓度显著高于开机前(P < 0.05)。ELISA分析结果显示,灰尘提取液中过敏原能与尘螨过敏性哮喘小鼠的IgE抗体产生结合反应。结论 芜湖地区居民空调器隔尘网中含有尘螨1类过敏原,可诱发哮喘。

关键词: 尘螨;过敏原;空调器隔尘网

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