Chin J Schisto Control ›› 2015, Vol. 27 ›› Issue (5): 470-.DOI: 10.16250/j.32.1374.2015082

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Expression of Tim? 3 on peripheral blood CD4 +T cells and its correlation with liver function damage parameters in advanced schistosomiasis patients

WU Qi-wen1 |ZHU Xiang1 |WANG Yu-ping1 | FU Xia1 | YANG Jin-sun2 |CAO Zhi-guo3*   

  1. 1 Department of Laboratory|First Affiliated Hospital of Wannan Medical College|Wuhu 241001|China;2 Department of Infec? tious Diseases|First Affiliated Hospital of Wannan Medical College|China;3 Anhui Provincial Institute of Schistosomiasis Con? trol|China
  • Online:2015-10-21 Published:2015-10-21

晚期血吸虫病患者外周血CD4+ T细胞Tim?3分子表达及与肝功能损伤指标的相关性

武其文1|朱翔1|王玉萍1|付夏1|杨进孙2|操治国3*   

  1. 1 皖南医学院第一附属医院检验科 (芜湖 241001); 2 皖南医学院第一附属医院感染性疾病科; 3 安徽省血吸虫病防治研究所
  • 通讯作者: 操治国
  • 作者简介:武其文| 男| 博士| 副主任医师。研究方向: 分子免疫学
  • 基金资助:
    皖南医学院第一附属医院人才引进基金(YR200904); 安徽省教育厅自然科学研究项目(KJ2010B247 )

Abstract: Objective Objective To explore the expression levels of T cell immunoglobulin and mucin domain protein 3(Tim?3)on peripheral blood CD4+ T cells and its correlation with liver function damage parameters in advanced schistosomiasis patients. Method Method Totally 28 advanced schistosomiasis patients were selected as study subjects,and 20 chronic schistosomiasis patients and 30 healthy persons were selected as controls. The expression levels of Tim?3 on CD4+ T cell were detected by flow cytome? try,and the serum IFN?γ and IL?4 levels were detected by ELISA. Hitachi 7600 biochemical analyzer was used to analyze the liver function parameters(ALT,γ?GT and TBIL) . Results Results There was a significant difference in Tim?3 expression among the three groups(F = 4.578, P < 0.05) . Tim?3 expression level in advanced schistosomiasis patients was(8.33 ± 2.28) %,which was significantly higher than that of the healthy control group(6.57 ± 1.99) %(t = 3.015,P < 0.01) . The Spearman nonparamet? ric correlation analysis showed that the Tim?3 expression level on CD4+ T cells in advanced schistosomiasis patients was positive? ly correlated with the serum ALT (rs = 0.746,P < 0.01) ,γ?GT (rs = 0.656,P < 0.01)and IL?4 (rs = 0.672,P < 0.01)levels, but negatively correlated with the serum IFN?γ levels(rs = -0.404,P < 0.05) . Conclusion Conclusion The expression of Tim?3 on pe? ripheral CD4+ T cells is increased in advanced schistosomiasis patients,which may regulate the function of CD4+ T cells and then be involved in the liver damage process of advanced schistosomiasis.

Key words: Advanced schistosomiasis; Liver damage; T cell immunoglobulin andmucin domain protein 3(Tim?3); CD4+ T cell

摘要: 目的 目的 检测T细胞免疫球蛋白域黏蛋白结构域分子3 (T cell immunoglobulin and mucin domain protein 3, Tim?3) 在晚期血吸虫病患者外周血CD4+ T细胞表面的表达, 并探讨其与肝功能损伤指标的关系。方法 方法 以28例晚期血吸虫病 患者作为研究对象, 同时选择20例慢性血吸虫病患者和30例健康人群作为对照, 采用流式细胞术检测CD4+ T细胞Tim?3 表达水平, 用ELISA法检测血清干扰素?γ (IFN?γ) 和白细胞介素?4 (IL?4) 水平, 以日立7600型生化分析仪检测肝功能指标 丙氨酸氨基转移酶 (ALT)、 γ?谷氨酰转移酶 (γ?GT) 和总胆红素 (TBIL) 浓度。 结果 结果 晚期血吸虫病、 慢性血吸虫病患者以 及健康人群外周血CD4+ T细胞表面Tim?3表达水平差异具有统计学意义 (F = 4.578, P < 0.05), 晚期血吸虫病患者外周血 CD4+ T细胞Tim?3表达水平为 (8.33 ± 2.28) %, 显著高于健康对照人群的 (6.57 ± 1.99) % (t = 3.015, P < 0.01)。Spearman 相关分析显示, 晚期血吸虫病患者外周血CD4+ T细胞Tim?3表达水平与患者血清ALT (rs = 0.746, P < 0.01)、 γ?GT (rs = 0.656, P < 0.01)、 IL?4水平 (rs = 0.672, P < 0.01) 呈正相关, 与IFN?γ水平 (rs = -0.404, P < 0.05) 呈负相关。 结论 结论 晚期 血吸虫病患者外周血CD4+ T细胞Tim?3表达上调, 其可能通过调节CD4+ T细胞功能参与晚期血吸虫病肝损伤过程。

关键词: 晚期血吸虫病; 肝损伤; Tim?3; CD4+ T细胞

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