Abstract: Objective To understand the endemic situation of Clonorchis sinensis in its second intermediate hosts in Pizhou and Xinyi cities of Jiangsu Province， so as to provide the evidence for the further control and treatment. Methods Pseudorasbora parva and Abbottina rivularis were caught from the natural water body of Pizhou and Xinyi cities， and the tabletting microscopy method was applied to test the metacercaria of C. sinensis. Results Totally 1 117 fishes were caught and dissected， including 792 P. parva （70.90%） and 325 A. rivularis （29.10%）. The metacercaria of C. sinensis infection rates of P. parva and A. rivularis were 29.80% （236/792） and 4.62% （15/325） respectively， and the difference between the two kinds of fishes was significant （[χ2]= 83.88， P＜0.01）. Conclusion The metacercaria of C. sinensis infection rate of freshwater fishes in Pizhou and Xinyi cities is high， and the local residents are facing the higher risk of clonorchiasis sinensis.

Key words: Clonorchis sinensis； Pseudorasbora parva； Abbottina rivularis； Epidemiological investigation, Pizhou City； Xinyi City

摘要： 目的 了解江苏省邳州市和新沂市华支睾吸虫在淡水鱼中的流行状况，为下一步的防治提供科学依据。 方法 在邳州市和新沂市自然水域中抓取野生麦穗鱼和棒花鱼，用压片镜检法检测鱼体内的华支睾吸虫囊蚴，分析感染数据。 结果 共解剖麦穗鱼和棒花鱼1 117条，其中麦穗鱼792条（占70.90%），棒花鱼325条（占29.10%）。麦穗鱼和棒花鱼华支睾吸虫囊蚴总感染率分别为29.80%（236/792）和4.62%（15/325），差异有统计学意义（[χ2]= 83.88， P＜0.01）。 结论 新沂市和邳州市淡水鱼华支睾吸虫囊蚴感染率较高，当地居民面临着较高的感染华支睾吸虫的风险。

关键词: 华支睾吸虫；麦穗鱼；棒花鱼；流行病学调查；邳州市；新沂市