关键词: 刚地弓形虫； HIV； GRA6； 基因型； 大理

Abstract: Objective Objective To comparatively analyze Toxoplasma gondii separated from HIV ? positive people and RH strain GRA6 gene. Methods Methods By using the nested PCR，the amplification of Dali HIV?positive blood samples and RH strains of Toxo? plasma GRA6 genome was performed. The GRA6 gene amplification positive product was selected and the electrophoresis imag? ing was performed by being digested with the Mse I endonuclease，and the gene sequences were measured and analyzed. Re Re? ? sults sults The GRA6 gene fragment（800 bp）was successfully amplified，and about 600 bp and 200 bp bands were got by Mse I. The sequencing results showed that T. gondii GRA6 gene positive samples had 2 nucleotide variation compared with T. gondii strain RH，namely 447 base pair at C becoming G，and 623 base pair at G becoming T. At 146 bp and 690 bp，the Mse I restric? tion sites（TTAA）were found. Conclusion Conclusion The preliminary judgment shows that the Dali HIV?positive T. gondii genotype is consistent with RH strain，belonging to genotype I.

Key words: Toxoplasma gondii；HIV；GRA6； Genotype；Dali