关键词: 恶性疟原虫； Pfcrt基因； K76T； 多态性

Abstract: Objective To identify the incidence of the K76T mutation in Pfcrt genes of imported Plasmodium falciparum. Methods The blood samples were collected from returnees infected with P. falciparum in endemic areas of Southeast Asia and Af? rica from 2008 to 2012. According to the Pfcrt gene sequence of P. falciparum，nested PCR primers were designed. Nested PCR? RFLP was applied with falciparum DNA in the blood samples as templates. Results Among 92 blood samples of P. falciparum， the mutant Pfcrt alleles were found in 50 samples（54.3%），and the wild type Pfcrt alleles were found in 42 samples（45.7%） . There were 33 samples（47.1%）with mutant Pfcrt alleles and 37 samples（52.9%）with wild type，respectively，from Africa. There were 17 samples（77.3%）with mutant Pfcrt alleles and 5 samples（22.7%）with wild type，respectively，from Southeast Asia. There was a significant difference between the Africa group and the Southeast Asia group（χ2 = 6.12，P < 0.05） . Conclu? sion The incidence of Pfcrt gene mutation is different among P. falciparum isolates from different regions. Therefore，Pfcrt K76T has an application value in the surveillance of the imported falciparum chloroquine? resistance.

Key words: Plasmodium falciparum；Pfcrt gene；K76T； Polymorphism