中国血吸虫病防治杂志 ›› 2010, Vol. 22 ›› Issue (1): 13-16.

• 论著 • 上一篇    下一篇

日本血吸虫可溶性成虫抗原和虫卵抗原对CD4+T 淋巴细胞凋亡和细胞周期的影响

张萃|陈晓军|朱继峰|迟莹|温小云|贺蕾|周莎|刘丰|苏川   

  1. 南京医科大学病原生物学系(南京 210029)
  • 出版日期:2010-02-15 发布日期:2010-03-10
  • 通讯作者: 苏川,男,教授。研究方向:血吸虫感染免疫
  • 作者简介:张萃|女|硕士研究生。研究方向:血吸虫感染免疫学
  • 基金资助:

    国家自然科学基金(30872206);江苏省高校自然科学基础研究项目(07KJA31023);江苏省自然科学基金(BK2007533);国家科技重大专项(2008ZX10004-011)

Effect of soluble worm antigen and soluble egg antigen of Schistosoma japonicum on apoptosis and cell-cycle of CD4+ T lymphocytes

Zhang Cui, Chen Xiao-jun, Zhu Ji-feng, Chi Ying, Wen Xiao-yun, He Lei, Zhou Sha, Liu Feng, Su Chuan   

  • Online:2010-02-15 Published:2010-03-10
  • Contact: Su Chuan, Male, Professor. Major: Immunology of schistosome infection

摘要:

[摘要目的 观察并比较日本血吸虫可溶性成虫抗原(SWA)、可溶性虫卵抗原(SEA)对小鼠CD4+T淋巴细胞凋亡和细胞周期的影响。方法 以免疫磁珠分选正常小鼠脾细胞中的CD4+T淋巴细胞后,与羧基荧光素二醋酸盐琥珀酰亚胺酯标记的抗原递呈细胞共培养,并分别以PBSSWASEA刺激。36 h以流式细胞术( FCM)结合caspase-3荧光抗体标记技术检测CD4+T淋巴细胞的凋亡水平;96 h以碘化丙锭染色结合FCM分析CD4+T淋巴细胞周期分布情况。结果 凋亡分析结果显示,经SEASWA刺激后,外周CD4+T细胞的凋亡百分比分别为(1.24±0.29%和(1.52±0.38%,二者差异无统计学意义(P>0.05)。细胞周期分析表明, SWA组处于G1期、S期和G2/M期的细胞百分比分别为(78.91±2.98%7.39±0.85%10.69±1.05%与之相比,SEAG1期细胞百分比[59.42±1.32%]显著降低,S[21.07±0.88%]G2/M[18.88±1.21%]显著增加,差异均有统计学意义(P0.01结论 SWASEA均可诱导CD4+T淋巴细胞凋亡;SEA促进CD4+T淋巴细胞进入分裂周期的作用更明显。

关键词: 日本血吸虫, 可溶性成虫抗原, 可溶性虫卵抗原, CD4+T 淋巴细胞, 凋亡, 细胞周期

Abstract:

[Abstract] Objective To investigate and compare the different effects of soluble adult worm antigen (SWA) and soluble egg antigen (SEA) of Schistosoma japonicum on the apoptosis and cell-cycle of murine CD4+ T cells. Methods  Purified CD4+T cells from normal C57BL/6 mice were cultured with CFSE labeled antigen presenting cells (APC) in the presence of different stimulus for 36 h. Flow cytometry (FCM) was used to detect the apoptosis of CD4+ T cells by fluorescence conjugated caspase-3 antibodie staining. The flow cytometry was used to analyze the cell-cycle of CD4+ T cells cultured as described above for 96 h by propidium iodide staining. Results Compared with the apoptosis percentage of CD4+ T cells[(1.24±0.29%] in the SEA stimulated group, that [(1.52±0.38)%] in the SWA stimulated group did not show statistically significant difference(P>0.05). Compared with the cell percentages in G1 phase[(78.91±2.98)%], S phase[(7.39±0.85)%] and G2/M phase[(10.69±1.05)%] in the SWA stimulated group, that of the G1 phase[(59.42±1.32)%] was significantly lower, but those in the S phase [(21.07±0.88)%] and G2/M phase[(18.88±1.21)%] were significantly increased in the SEA stimulated groupP0.01. Conclusions There is no statistically significant difference between the apoptosis levels of CD4+T cells stimulated by SWA and SEA. However, SEA significantly promotes the progression of the cell-cycle of CD4+T cells compared with SWA.

Key words: Schistosoma japonicum, Soluble worm antigen(SWA), Soluble egg antigen(SEA), CD4+ T cell, Apoptosis, Cell-cycle